Overview

  • Product name

    Anti-PLA2R antibody [12-6-5]
    See all PLA2R primary antibodies
  • Description

    Mouse monoclonal [12-6-5] to PLA2R
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human PLA2R aa 20-663. corresponding to extracellular sequence NC3 (containing domains N-terminal Cysteine rich-FibII-CTLD1-CTLD2-CTLD3) expressed in HEK 293-EBNA-1 cells
    Sequence:

    A EGVAAALTPE RLLEWQDKGI FVIQSESLKK CIQAGKSVLT LENCKQANKH MLWKWVSNHG LFNIGGSGCL GLNFSAPEQP LSLYECDSTL VSLRWRCNRK MITGPLQYSV QVAHDNTVVA SRKYIHKWIS YGSGGGDICE YLHKDLHTIK GNTHGMPCMF PFQYNHQWHH ECTREGREDD LLWCATTSRY ERDEKWGFCP DPTSAEVGCD TIWEKDLNSH ICYQFNLLSS LSWSEAHSSC QMQGGTLLSI TDETEENFIR EHMSSKTVEV WMGLNQLDEH AGWQWSDGTP LNYLNWSPEV NFEPFVEDHC GTFSSFMPSA WRSRDCESTL PYICKKYLNH IDHEIVEKDA WKYYATHCEP GWNPYNRNCY KLQKEEKTWH EALRSCQADN SALIDITSLA EVEFLVTLLG DENASETWIG LSSNKIPVSF EWSNDSSVIF TNWHTLEPHI FPNRSQLCVS AEQSEGHWKV KNCEERLFYI CKKAGHVLSD AESGCQEGWE RHGGFCYKID TVLRSFDQAS SGYYCPPALV TITNRFEQAF ITSLISSVVK MKDSYFWIAL QDQNDTGEYT WKPVGQKPEP VQYTHWNTHQ PRYSGGCVAM RGRHPLGRWE VKHCRHFKAM SLCKQPVENQ EKAEYEERWP FHP


    Database link: Q13018

  • Positive control

    • WB: Recombinant extracellular domain (NC8) and N terminal fragment (NC3; immunogen) of human PLA2R. ICC/IF: PLA2R over-expressing podocytes. IHC-P: Normal human kidney and membranous nephropathy kidney tissues. Flow Cytometry: PLA2R over-expressing podocytes.

Properties

Applications

Our Abpromise guarantee covers the use of ab211490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Predicted molecular weight: 169 kDa.
Flow Cyt 1/200.
ICC/IF 1/400.
IHC-P 1/5000 - 1/20000.

Perform heat mediated antigen retrieval at pH 8 before commencing with IHC staining protocol. See attached document for protocol details.

Target

  • Function

    Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phosholipase sPLA2-IB/PLA2G1B but not sPLA2-IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in proinflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B.
  • Tissue specificity

    Present in lung macrophage (at protein level). Highly expressed in kidney. Also expressed in pancreas, amnion, choriodecidua and placenta. Isoform 2 is expressed at much lower level.
  • Sequence similarities

    Contains 8 C-type lectin domains.
    Contains 1 fibronectin type-II domain.
    Contains 1 ricin B-type lectin domain.
  • Domain

    C-type lectin domains 3-5 mediate the interaction with phospholipase PLA2G1B.
    The endocytosis signal probably mediates endocytosis via clathrin-coated pits.
  • Post-translational
    modifications

    The secretory phospholipase A2 receptor form may be produced by the action of metalloproteinases. It contains all extracellular domains and only lacks transmembrane and cytosolic regions. It is however unclear whether this form is produced by proteolytic cleavage as suggested by some experiments, or by alternative splicing, as in the case of isoform 2 that shares all characteristics of secretory phospholipase A2 receptor form.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 180 kDa secretory phospholipase A2 receptor antibody
    • C-type lectin domain family 13 member C antibody
    • CLEC13C antibody
    • M type receptor antibody
    • M-type receptor antibody
    • Phospholipase A2 receptor 1 antibody
    • PLA2 R antibody
    • PLA2-R antibody
    • PLA2G1R antibody
    • PLA2IR antibody
    • PLA2R antibody
    • PLA2R_HUMAN antibody
    • PLA2R1 antibody
    • Soluble PLA2-R antibody
    • Soluble PLA2R antibody
    • Soluble secretory phospholipase A2 receptor antibody
    see all

Images

  • All lanes : Anti-PLA2R antibody [12-6-5] (ab211490) at 1/10000 dilution

    Lane 1 : Recombinant extracellular domain of human PLA2R (NC8)
    Lane 2 : Recombinant N-terminus fragment of human PLA2R (NC3)

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 169 kDa

  • Immunohistochemical analysis of membranous nephropathy kidney tissue labeling PLA2R at 1/5000 dilution followed by DAB detection.

  • Immunofluorescent analysis of PLA2R over-expressing podocytes labeling PLA2R at 1/400 dilution (green).

  • Flow cytometry analysis of wild type (dark blue line) and over-expressing PLA2R podocytes (low expressor, green line; high expressor, red line) labeling PLA2R using ab211490 at 1/200 dilution. Negative controls were unlabelled cells (turquoise line) and mouse IgG (grey line).

  • Immunohistochemical analysis of normal human kidney tissue labeling PLA2R at 1/5000 dilution followed by DAB detection.

  • Immunohistochemical analysis of normal human kidney tissue labeling PLA2R at 1/5000 dilution followed by DAB detection.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab211490.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up