• Product name

  • Description

    Rabbit polyclonal to PLA2R
  • Host species

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Predicted to work with: Rabbit, Cow, Orangutan
  • Immunogen

    Recombinant fragment corresponding to Human PLA2R aa 395-530.


    Database link: Q13018

  • Positive control

    • Human lymph node tissue; Human cell line U-2 OS.



Our Abpromise guarantee covers the use of ab194256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.
WB Use a concentration of 0.4 µg/ml. Predicted molecular weight: 169 kDa.


  • Function

    Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phosholipase sPLA2-IB/PLA2G1B but not sPLA2-IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in proinflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B.
  • Tissue specificity

    Present in lung macrophage (at protein level). Highly expressed in kidney. Also expressed in pancreas, amnion, choriodecidua and placenta. Isoform 2 is expressed at much lower level.
  • Sequence similarities

    Contains 8 C-type lectin domains.
    Contains 1 fibronectin type-II domain.
    Contains 1 ricin B-type lectin domain.
  • Domain

    C-type lectin domains 3-5 mediate the interaction with phospholipase PLA2G1B.
    The endocytosis signal probably mediates endocytosis via clathrin-coated pits.
  • Post-translational

    The secretory phospholipase A2 receptor form may be produced by the action of metalloproteinases. It contains all extracellular domains and only lacks transmembrane and cytosolic regions. It is however unclear whether this form is produced by proteolytic cleavage as suggested by some experiments, or by alternative splicing, as in the case of isoform 2 that shares all characteristics of secretory phospholipase A2 receptor form.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 180 kDa secretory phospholipase A2 receptor antibody
    • C-type lectin domain family 13 member C antibody
    • CLEC13C antibody
    • M type receptor antibody
    • M-type receptor antibody
    • Phospholipase A2 receptor 1 antibody
    • PLA2 R antibody
    • PLA2-R antibody
    • PLA2G1R antibody
    • PLA2IR antibody
    • PLA2R antibody
    • PLA2R_HUMAN antibody
    • PLA2R1 antibody
    • Soluble PLA2-R antibody
    • Soluble PLA2R antibody
    • Soluble secretory phospholipase A2 receptor antibody
    see all


  • Western blot analysis in human kidney tissue staining PLA2R with ab194256.

  • Immunohistochemical analysis of paraffin-embedded Human lymph node tissue sections labeling PLA2R with ab194256 at a 1/500 dilution.

  • Immunofluorescence analysis of Human cell line U-2OS labeling PLA2R with ab194256 at 4 µg/mL. Fixation/permeabilization: PFA/Triton X-100.


ab194256 has not yet been referenced specifically in any publications.

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