Key features and details
- Rabbit polyclonal to PLA2R
- Suitable for: IHC-Fr, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PLA2R antibody
See all PLA2R primary antibodies
DescriptionRabbit polyclonal to PLA2R
Tested applicationsSuitable for: IHC-Fr, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Cow
Synthetic peptide derived from the C terminal domain of human PLA2R
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 23rd June 2017. The following lots are still unpurified and still in stock as of 23rd June 2017 - GR3173938-1, GR3173938-3, GR3173938-4, GR3173938-5, GR306201-4, GR306201-5, GR3173938-2, GR306201-3, GR306201-2, GR281777-4. Lot numbers other than GR3173938-1, GR3173938-3, GR3173938-4, GR3173938-5, GR306201-4, GR306201-5, GR3173938-2, GR306201-3, GR306201-2, GR281777-4 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferConstituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose
Concentration information loading...
PurityProtein A purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab80054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/100 - 1/1000.|
|WB||1/500 - 1/5000. Predicted molecular weight: 169 kDa.Can be blocked with PLA2R peptide (ab176432).|
FunctionReceptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phosholipase sPLA2-IB/PLA2G1B but not sPLA2-IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in proinflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B.
Tissue specificityPresent in lung macrophage (at protein level). Highly expressed in kidney. Also expressed in pancreas, amnion, choriodecidua and placenta. Isoform 2 is expressed at much lower level.
Sequence similaritiesContains 8 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain.
DomainC-type lectin domains 3-5 mediate the interaction with phospholipase PLA2G1B.
The endocytosis signal probably mediates endocytosis via clathrin-coated pits.
modificationsThe secretory phospholipase A2 receptor form may be produced by the action of metalloproteinases. It contains all extracellular domains and only lacks transmembrane and cytosolic regions. It is however unclear whether this form is produced by proteolytic cleavage as suggested by some experiments, or by alternative splicing, as in the case of isoform 2 that shares all characteristics of secretory phospholipase A2 receptor form.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- 180 kDa secretory phospholipase A2 receptor antibody
- C-type lectin domain family 13 member C antibody
- CLEC13C antibody
ab80054 has been referenced in 3 publications.
- Ramachandran R et al. Membranous Nephropathy With Light Chain Restricted Deposits. Nephrology (Carlton) N/A:N/A (2018). PubMed: 29633425
- Ramachandran R et al. PLA2R antibodies, glomerular PLA2R deposits and variations in PLA2R1 and HLA-DQA1 genes in primary membranous nephropathy in South Asians. Nephrol Dial Transplant 31:1486-93 (2016). PubMed: 26673907
- Kumar V et al. Antibodies to m-type phospholipase A2 receptor in children with idiopathic membranous nephropathy. Nephrology (Carlton) 20:572-5 (2015). PubMed: 26194981