• Product name

  • Description

    Goat polyclonal to PLA2R
  • Host species

  • Specificity

    ab99466 is expected to recognize both isoforms (NP_031392.3; NP_001007268.1).
  • Tested applications

    Suitable for: IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Dog, Human, Pig
    Predicted to work with: Rabbit, Cow, Orangutan
  • Immunogen

    Synthetic peptide:


    , corresponding to internal sequence amino acids 946-957 of Human PLA2R (NP_031392.3; NP_001007268.1).

  • Positive control

    • Human Ileum lysate.



Our Abpromise guarantee covers the use of ab99466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 153 kDa.


  • Function

    Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phosholipase sPLA2-IB/PLA2G1B but not sPLA2-IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in proinflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B.
  • Tissue specificity

    Present in lung macrophage (at protein level). Highly expressed in kidney. Also expressed in pancreas, amnion, choriodecidua and placenta. Isoform 2 is expressed at much lower level.
  • Sequence similarities

    Contains 8 C-type lectin domains.
    Contains 1 fibronectin type-II domain.
    Contains 1 ricin B-type lectin domain.
  • Domain

    C-type lectin domains 3-5 mediate the interaction with phospholipase PLA2G1B.
    The endocytosis signal probably mediates endocytosis via clathrin-coated pits.
  • Post-translational

    The secretory phospholipase A2 receptor form may be produced by the action of metalloproteinases. It contains all extracellular domains and only lacks transmembrane and cytosolic regions. It is however unclear whether this form is produced by proteolytic cleavage as suggested by some experiments, or by alternative splicing, as in the case of isoform 2 that shares all characteristics of secretory phospholipase A2 receptor form.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 180 kDa secretory phospholipase A2 receptor antibody
    • C-type lectin domain family 13 member C antibody
    • CLEC13C antibody
    • M type receptor antibody
    • M-type receptor antibody
    • Phospholipase A2 receptor 1 antibody
    • PLA2 R antibody
    • PLA2-R antibody
    • PLA2G1R antibody
    • PLA2IR antibody
    • PLA2R antibody
    • PLA2R_HUMAN antibody
    • PLA2R1 antibody
    • Soluble PLA2-R antibody
    • Soluble PLA2R antibody
    • Soluble secretory phospholipase A2 receptor antibody
    see all


  • ab99466 staining PLA2R in dog kidney tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked for 10 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + BSA) for 17 hours at 4°C. An Alexa Fluor® 568-conjugated rabbit anti-goat IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Western Blot analysis showing approximate 150kDa band observed in Human Kidney and Pig Lung lysates.

  • Anti-PLA2R antibody (ab99466) at 1 µg/ml + Human Ileum lysate at 35 µg

    Predicted band size: 153 kDa


ab99466 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Frozen sections)
Blocking step
Block Ace as blocking agent for 10 minute(s) · Concentration: 4% · Temperature: 25°C
Dog Tissue sections (kidney)

Abcam user community

Verified customer

Submitted Jun 23 2014


Thank you for contacting us and your interest in our products.

Unfortunately, we do not currently have any antibodies directed against PLA2R which have shown to detect the protein in mouse samples. I have checked the immunogen used to raise the two antibodies we have against this target (ab80054 and ab99466). Both unfortunately share very low sequence homology with the mouse protein, we would therefore not expect the antibodies to cross react.

I am sorry that I could not be of more help in this instance. If you have any further questions, please do not hesitate to ask.

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Thank you for contacting us. I can confirm that the immunogen sequences for these two antibodies are different. The immunogen for ab99466 is from an internal domain (amino acid 946 - 957), whereas the immunogen for ab80054 is from a C terminal domain (exact sequence is however proprietary information). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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