Anti-Placental alkaline phosphatase (PLAP) antibody [8B6] (ab33)

Mouse monoclonal Placental alkaline phosphatase (PLAP) antibody [8B6]. Validated in ELISA, IHC, RIA and tested in Human. Cited in 5 publication(s). Immunogen corresponding to tissue, cells or virus.


  • Product name

    Anti-Placental alkaline phosphatase (PLAP) antibody [8B6]
    See all Placental alkaline phosphatase (PLAP) primary antibodies
  • Description

    Mouse monoclonal [8B6] to Placental alkaline phosphatase (PLAP)
  • Host species

  • Tested applications

    Suitable for: ELISA, IHC-P, RIA, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Placental alkaline phosphatase (PLAP). Fresh Hep-2 cells followed by booster injections of fresh Hep-2 cells that have had their surface expression of PLAP boosted in culture.

  • Positive control

    • IHC-Fr: Normal human placenta tissue sections.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: 0.02% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

  • Clone number

  • Myeloma

  • Isotype

  • Light chain type

  • Research areas


Our Abpromise guarantee covers the use of ab33 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.
RIA Use at an assay dependent concentration.
IHC-Fr 1/250 - 1/500.


  • Sequence similarities

    Belongs to the alkaline phosphatase family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alkaline phosphatase antibody
    • Alkaline phosphatase placental antibody
    • Alkaline phosphatase placental type antibody
    • Alkaline phosphatase Regan isozyme antibody
    • ALP antibody
    • Alp1 antibody
    • ALPP antibody
    • FLJ61142 antibody
    • Germ-cell alkaline phosphatase antibody
    • nagao isozyme antibody
    • OTTHUMP00000164354 antibody
    • PALP antibody
    • Placental alkaline phosphatase 1 antibody
    • placental heat-stable alkaline phosphatase antibody
    • placental type antibody
    • PLAP antibody
    • PLAP-1 antibody
    • PLAP1 antibody
    • PPB1_HUMAN antibody
    see all


  • IHC image of Placental alkaline phosphatase staining in a section of frozen normal human placenta, fixed in 10% paraformaldehyde (10 min).

    Staining was performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab33, 10 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

    The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:

  • Müller WEG  et al. Polyphosphate as a donor of high-energy phosphate for the synthesis of ADP and ATP. J Cell Sci 130:2747-2756 (2017). Read more (PubMed: 28687622) »
  • Sharp TM  et al. Inhibition of cellular protein secretion by norwalk virus nonstructural protein p22 requires a mimic of an endoplasmic reticulum export signal. PLoS One 5:e13130 (2010). ICC/IF ; Human . Read more (PubMed: 20976190) »
See all 5 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A


Thank you for your enquiry. Unfortunately we have not tested ab33 against mouse so we do not know whether it is suitable. The antibody was raised against the whole protein, and although the mouse and human protein show 75% homology we do not know which part of the protein it is reactive against and thus the exact homology in mouse. We do stock another antibody which is reactive in IHC-P on mouse samples, which would be more suitable: ab16695 - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] IHC-P, WB Reacts with Mouse and Human It has not been tested in ICC on primary cultures, but if you are interested in testing it as part of our program, just let me know. I hope this information helps.

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Thank you for your enquiry. According to the database SwissProt the MW of the precursor PLAP is 57kDa and it is glycosylated therefore its weight should be higher than this. A paper by Webb et al (Placenta. 1986 Sep-Oct;7(5):405-15.Isolation of placental-type alkaline phosphatase associated with human syncytiotrophoblast membranes using monoclonal antibodies) describes "an eluted enzymatically active PLAP had a molecular weight in the regions of 115K and 130K, with an occasional higher molecular weight form at 180K. On reduction to a 66K monomeric form". Regarding dilutions to try, I would recommend trying a range of dilutions in a preliminary experiment (1:250, 1:500, 1:1000) with overnight incubation and optimising following the results of that experiment. If the customer decides to go ahead and purchase this product, please ask him/her to let us know how he/she gets on by submitting an Abreview and in return we will award him/her 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image). Please let me know if you require further information,

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I'm afraid that we do not have any data regarding the suitablity of this antibody for WB.

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This antibody has not been tested in flow cytometry.

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This antibody has not been tested in immunofluorescence.

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This monoclonal was raised against the whole protein, and in the absence of any epitope mapping studies, I am afraid that we do not know against which part of the protein the antibody is reactive.

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Vlad We do not have any data on this antibody's inhibiting activity in vivo or in vitro. We are about to implement a trial 'try risk free' scheme which says that if an antibody is purchased and used in an experiment outside that written on the datasheet, and it is found not to work, in return for the negative data (which we can add to the datasheet to help other researchers) we will refund or issue a credit note.

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This antibody has not yet been tested in Western blotting.

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