Overview

  • Product name
    Anti-Placental alkaline phosphatase (PLAP) antibody [8B6]
    See all Placental alkaline phosphatase (PLAP) primary antibodies
  • Description
    Mouse monoclonal [8B6] to Placental alkaline phosphatase (PLAP)
  • Host species
    Mouse
  • Specificity
    Human placental alkaline phosphatase, Regan and Nagao isoenzymes. No cross reaction with other isoenzymes of AP.
  • Tested applications
    Suitable for: ELISA, IHC-P, RIA, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    HEp-2 cells with boosted surface expression of PLAP

  • Positive control
    • Placenta.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    8B6
  • Myeloma
    P3-NS1/1-Ag4-1
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P 1/250 - 1/500.
RIA Use at an assay dependent concentration.
IHC-Fr 1/250 - 1/500.

Target

  • Sequence similarities
    Belongs to the alkaline phosphatase family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alkaline phosphatase antibody
    • Alkaline phosphatase placental antibody
    • Alkaline phosphatase placental type antibody
    • Alkaline phosphatase Regan isozyme antibody
    • ALP antibody
    • Alp1 antibody
    • ALPP antibody
    • FLJ61142 antibody
    • Germ-cell alkaline phosphatase antibody
    • nagao isozyme antibody
    • OTTHUMP00000164354 antibody
    • PALP antibody
    • Placental alkaline phosphatase 1 antibody
    • placental heat-stable alkaline phosphatase antibody
    • placental type antibody
    • PLAP antibody
    • PLAP-1 antibody
    • PLAP1 antibody
    • PPB1_HUMAN antibody
    see all

References

This product has been referenced in:
  • Sharp TM  et al. Inhibition of cellular protein secretion by norwalk virus nonstructural protein p22 requires a mimic of an endoplasmic reticulum export signal. PLoS One 5:e13130 (2010). ICC/IF ; Human . Read more (PubMed: 20976190) »
  • Zhang L  et al. Alteration in the IL-2 signal peptide affects secretion of proteins in vitro and in vivo. J Gene Med 7:354-65 (2005). ELISA ; Human . Read more (PubMed: 15619290) »
See all 4 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer

Thank you for your enquiry. Unfortunately we have not tested ab33 against mouse so we do not know whether it is suitable. The antibody was raised against the whole protein, and although the mouse and human protein show 75% homology we do not know which part of the protein it is reactive against and thus the exact homology in mouse. We do stock another antibody which is reactive in IHC-P on mouse samples, which would be more suitable: ab16695 - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] IHC-P, WB Reacts with Mouse and Human It has not been tested in ICC on primary cultures, but if you are interested in testing it as part of our https://www.abcam.com/abtrial program, just let me know. I hope this information helps.

Read More

Answer

Thank you for your enquiry. According to the database SwissProt the MW of the precursor PLAP is 57kDa and it is glycosylated therefore its weight should be higher than this. A paper by Webb et al (Placenta. 1986 Sep-Oct;7(5):405-15.Isolation of placental-type alkaline phosphatase associated with human syncytiotrophoblast membranes using monoclonal antibodies) describes "an eluted enzymatically active PLAP had a molecular weight in the regions of 115K and 130K, with an occasional higher molecular weight form at 180K. On reduction to a 66K monomeric form". Regarding dilutions to try, I would recommend trying a range of dilutions in a preliminary experiment (1:250, 1:500, 1:1000) with overnight incubation and optimising following the results of that experiment. If the customer decides to go ahead and purchase this product, please ask him/her to let us know how he/she gets on by submitting an Abreview and in return we will award him/her 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image). Please let me know if you require further information,

Read More

Answer

I'm afraid that we do not have any data regarding the suitablity of this antibody for WB.

Read More

Question
Answer

This antibody has not been tested in flow cytometry.

Read More

Answer

This antibody has not been tested in immunofluorescence.

Read More

Answer

This monoclonal was raised against the whole protein, and in the absence of any epitope mapping studies, I am afraid that we do not know against which part of the protein the antibody is reactive.

Read More

Answer

Vlad We do not have any data on this antibody's inhibiting activity in vivo or in vitro. We are about to implement a trial 'try risk free' scheme which says that if an antibody is purchased and used in an experiment outside that written on the datasheet, and it is found not to work, in return for the negative data (which we can add to the datasheet to help other researchers) we will refund or issue a credit note.

Read More

Answer

This antibody has not yet been tested in Western blotting.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up