Recombinant Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
![Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)](https://www.abcam.com/ps/products/133/ab133602/Images/ab133602-383512-anti-placental-alkaline-phosphatase-plap-antibody-epr6141-western-blot-hela-human-placenta-human-heart.jpg "Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6141] to Placental alkaline phosphatase (PLAP)
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141]
See all Placental alkaline phosphatase (PLAP) primary antibodies -
Description
Rabbit monoclonal [EPR6141] to Placental alkaline phosphatase (PLAP) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Placental alkaline phosphatase (PLAP) aa 150-250 (extracellular). The exact sequence is proprietary.
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Positive control
- WB: Human placenta, and seminoma tissue lysates, and HeLa cell lysate. IHC-P: Human dysgerminoma and placenta tissues.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.10 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6141 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 58 kDa). | |
| IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Sequence similarities
Belongs to the alkaline phosphatase family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 250 Human
- Omim: 171800 Human
- SwissProt: P05187 Human
- Unigene: 284255 Human
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Alternative names
- Alkaline phosphatase antibody
- Alkaline phosphatase placental antibody
- Alkaline phosphatase placental type antibody
see all
Images
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Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)All lanes : Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ALPP knockout HeLa cell lysate
Lane 3 : Human placenta tissue lysate
Lane 4 : Human heart tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab133602 observed at 70 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab133602 Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] was shown to specifically react with Placental alkaline phosphatase (PLAP) in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261763 (knockout cell lysate ab256836) was used. Wild-type and Placental alkaline phosphatase (PLAP) knockout samples were subjected to SDS-PAGE. ab133602 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)](https://www.abcam.com/ps/products/133/ab133602/Images/ab133602-147443-anti-placental-alkaline-phosphatase-plap-antibody-epr6141-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling Placental alkaline phosphatase (PLAP) with ab133602 at 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)](https://www.abcam.com/ps/products/133/ab133602/Images/ab133602-147445-anti-placental-alkaline-phosphatase-plap-antibody-epr6141-western-blot.jpg)
Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)All lanes : Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602) at 1/10000 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : Human seminoma tissue lysate
Lane 3 : HeLa cell lysate at 10 µg
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 58 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)](https://www.abcam.com/ps/products/133/ab133602/Images/ab133602-147439-anti-placental-alkaline-phosphatase-plap-antibody-epr6141-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)ab133602 showing positive staining in Normal placenta tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)](https://www.abcam.com/ps/products/133/ab133602/Images/ab133602-147444-anti-placental-alkaline-phosphatase-plap-antibody-epr6141-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)Immunohistochemical analysis of paraffin-embedded Human dysgerminoma tissue labelling Placental alkaline phosphatase (PLAP) with ab133602 at 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)](https://www.abcam.com/ps/products/133/ab133602/Images/ab133602-147442-anti-placental-alkaline-phosphatase-plap-antibody-epr6141-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)ab133602 showing negative staining in Normal brain tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)](https://www.abcam.com/ps/products/133/ab133602/Images/ab133602-147441-anti-placental-alkaline-phosphatase-plap-antibody-epr6141-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)ab133602 showing negative staining in Normal liver tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)](https://www.abcam.com/ps/products/133/ab133602/Images/ab133602-147440-anti-placental-alkaline-phosphatase-plap-antibody-epr6141-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)ab133602 showing positive staining in Seminoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)](https://www.abcam.com/ps/products/133/ab133602/Images/ab133602-147438-anti-placental-alkaline-phosphatase-plap-antibody-epr6141-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)ab133602 showing negative staining in Normal colon tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)](https://www.abcam.com/ps/products/133/ab133602/Images/ab133602-147437-anti-placental-alkaline-phosphatase-plap-antibody-epr6141-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)ab133602 showing negative staining in Normal tonsil tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)](https://www.abcam.com/ps/products/133/ab133602/Images/ab133602-147436-anti-placental-alkaline-phosphatase-plap-antibody-epr6141.jpg)
OI-RD Scanning - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602) -
![Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)](https://www.abcam.com/ps/products/133/ab133602/Images/ab133602-13-benefits-of-recombinant-antibodies.png)
Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
Protocols
References (12)
ab133602 has been referenced in 12 publications.
- Lei P et al. Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis. J Orthop Surg Res 14:80 (2019). PubMed: 30885228
- Bing T et al. Aptameric Probe Specifically Binding Protein Heterodimer Rather Than Monomers. Adv Sci (Weinh) 6:1900143 (2019). PubMed: 31179220
- Xu E et al. Asymmetric expression of GPR126 in the convex/concave side of the spine is associated with spinal skeletal malformation in adolescent idiopathic scoliosis population. Eur Spine J 28:1977-1986 (2019). PubMed: 31079250
- Zhan JK et al. AMPK/TSC2/mTOR pathway regulates replicative senescence of human vascular smooth muscle cells. Exp Ther Med 16:4853-4858 (2018). PubMed: 30542441
- Hirschmugl B et al. Relation of placental alkaline phosphatase expression in human term placenta with maternal and offspring fat mass. Int J Obes (Lond) N/A:N/A (2018). PubMed: 29899523
