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Tags & Cell Markers Fusion / Marker Proteins Alkaline Phosphatase
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (12)

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Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
  • Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
  • OI-RD Scanning - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
  • Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6141] to Placental alkaline phosphatase (PLAP)
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141]
    See all Placental alkaline phosphatase (PLAP) primary antibodies
  • Description

    Rabbit monoclonal [EPR6141] to Placental alkaline phosphatase (PLAP)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Placental alkaline phosphatase (PLAP) aa 150-250 (extracellular). The exact sequence is proprietary.

  • Positive control

    • WB: Human placenta, and seminoma tissue lysates, and HeLa cell lysate. IHC-P: Human dysgerminoma and placenta tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 5.10 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6141
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • Alkaline Phosphatase

Associated products

  • Alternative Versions

    • HRP Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab198554)
    • Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] - BSA and Azide free (ab220315)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human ALPP (PLAP) knockout HeLa cell line (ab261763)
  • KO cell lysates

    • Human ALPP (PLAP) knockout HeLa cell lysate (ab256836)
  • Positive Controls

    • HeLa membrane extract lysate (ab29547)
  • Recombinant Protein

    • Recombinant Human Placental alkaline phosphatase (PLAP) protein (ab191941)

Applications

Our Abpromise guarantee covers the use of ab133602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 58 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Sequence similarities

      Belongs to the alkaline phosphatase family.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P05187 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 250 Human
      • Omim: 171800 Human
      • SwissProt: P05187 Human
      • Unigene: 284255 Human
      • Alternative names

        • Alkaline phosphatase antibody
        • Alkaline phosphatase placental antibody
        • Alkaline phosphatase placental type antibody
        • Alkaline phosphatase Regan isozyme antibody
        • ALP antibody
        • Alp1 antibody
        • ALPP antibody
        • FLJ61142 antibody
        • Germ-cell alkaline phosphatase antibody
        • nagao isozyme antibody
        • OTTHUMP00000164354 antibody
        • PALP antibody
        • Placental alkaline phosphatase 1 antibody
        • placental heat-stable alkaline phosphatase antibody
        • placental type antibody
        • PLAP antibody
        • PLAP-1 antibody
        • PLAP1 antibody
        • PPB1_HUMAN antibody
        see all

      Images

      • Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
        Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
        All lanes : Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602) at 1/1000 dilution

        Lane 1 : Wild-type HeLa cell lysate
        Lane 2 : ALPP knockout HeLa cell lysate
        Lane 3 : Human placenta tissue lysate
        Lane 4 : Human heart tissue lysate

        Lysates/proteins at 20 µg per lane.

        Performed under reducing conditions.

        Predicted band size: 58 kDa
        Observed band size: 70 kDa
        why is the actual band size different from the predicted?



        Lanes 1-4: Merged signal (red and green). Green - ab133602 observed at 70 kDa. Red - loading control, ab8245 observed at 37 kDa.

        ab133602 Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] was shown to specifically react with Placental alkaline phosphatase (PLAP) in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261763 (knockout cell lysate ab256836) was used. Wild-type and Placental alkaline phosphatase (PLAP) knockout samples were subjected to SDS-PAGE. ab133602 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

        Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling Placental alkaline phosphatase (PLAP) with ab133602 at 1/250.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
        Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
        All lanes : Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602) at 1/10000 dilution

        Lane 1 : Human placenta tissue lysate
        Lane 2 : Human seminoma tissue lysate
        Lane 3 : HeLa cell lysate at 10 µg

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 58 kDa
        Observed band size: 70 kDa why is the actual band size different from the predicted?

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

        ab133602 showing positive staining in Normal placenta tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

        Immunohistochemical analysis of paraffin-embedded Human dysgerminoma tissue labelling Placental alkaline phosphatase (PLAP) with ab133602 at 1/250.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

        ab133602 showing negative staining in Normal brain tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

        ab133602 showing negative staining in Normal liver tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

        ab133602 showing positive staining in Seminoma tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

        ab133602 showing negative staining in Normal colon tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

        ab133602 showing negative staining in Normal tonsil tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • OI-RD Scanning - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
        OI-RD Scanning - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)
        Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (12)

      Publishing research using ab133602? Please let us know so that we can cite the reference in this datasheet.

      ab133602 has been referenced in 12 publications.

      • Lei P  et al. Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis. J Orthop Surg Res 14:80 (2019). PubMed: 30885228
      • Bing T  et al. Aptameric Probe Specifically Binding Protein Heterodimer Rather Than Monomers. Adv Sci (Weinh) 6:1900143 (2019). PubMed: 31179220
      • Xu E  et al. Asymmetric expression of GPR126 in the convex/concave side of the spine is associated with spinal skeletal malformation in adolescent idiopathic scoliosis population. Eur Spine J 28:1977-1986 (2019). PubMed: 31079250
      • Zhan JK  et al. AMPK/TSC2/mTOR pathway regulates replicative senescence of human vascular smooth muscle cells. Exp Ther Med 16:4853-4858 (2018). PubMed: 30542441
      • Hirschmugl B  et al. Relation of placental alkaline phosphatase expression in human term placenta with maternal and offspring fat mass. Int J Obes (Lond) N/A:N/A (2018). PubMed: 29899523
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (RIPCreZ/HPAP pancreas)
      Specification
      RIPCreZ/HPAP pancreas
      Blocking step
      Sniper as blocking agent for 8 minute(s) · Concentration: 100% · Temperature: 21°C
      Fixative
      Paraformaldehyde
      Read More

      Ms. Christine Beamish

      Verified customer

      Submitted Sep 17 2013

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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