Name: Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141]
Brand: RabMAb
SKU: ab133602
Rating: 4 (2 reviews)

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Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6141] to Placental alkaline phosphatase (PLAP)
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Carrier Free HRP

Product name

Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141]
See all Placental alkaline phosphatase (PLAP) primary antibodies
Description

Rabbit monoclonal [EPR6141] to Placental alkaline phosphatase (PLAP)
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide within Human Placental alkaline phosphatase (PLAP) aa 150-250 (extracellular). The exact sequence is proprietary.
Positive control
- WB: Human placenta, and seminoma tissue lysates, and HeLa cell lysate. IHC-P: Human dysgerminoma and placenta tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 5.10 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6141
Isotype

IgG
Research areas

Alternative Versions
- HRP Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab198554)
- Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] - BSA and Azide free (ab220315)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human ALPP (PLAP) knockout HeLa cell line (ab261763)
KO cell lysates
- Human ALPP (PLAP) knockout HeLa cell lysate (ab256836)
Positive Controls
- HeLa membrane extract lysate (ab29547)
Recombinant Protein
- Recombinant Human Placental alkaline phosphatase (PLAP) protein (ab191941)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(1)	1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 58 kDa).
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
WB 1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 58 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes

Is unsuitable for Flow Cyt,ICC or IP.

Sequence similarities

Belongs to the alkaline phosphatase family.
Cellular localization

Cell membrane.
Target information above from: UniProt accession P05187 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 250 Human
- Omim: 171800 Human
- SwissProt: P05187 Human
- Unigene: 284255 Human
Alternative names
- Alkaline phosphatase antibody
- Alkaline phosphatase placental antibody
- Alkaline phosphatase placental type antibody
- Alkaline phosphatase Regan isozyme antibody
- ALP antibody
- Alp1 antibody
- ALPP antibody
- FLJ61142 antibody
- Germ-cell alkaline phosphatase antibody
- nagao isozyme antibody
- OTTHUMP00000164354 antibody
- PALP antibody
- Placental alkaline phosphatase 1 antibody
- placental heat-stable alkaline phosphatase antibody
- placental type antibody
- PLAP antibody
- PLAP-1 antibody
- PLAP1 antibody
- PPB1_HUMAN antibody
see all

Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

All lanes : Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ALPP knockout HeLa cell lysate
Lane 3 : Human placenta tissue lysate
Lane 4 : Human heart tissue lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 58 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab133602 observed at 70 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab133602 Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] was shown to specifically react with Placental alkaline phosphatase (PLAP) in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261763 (knockout cell lysate ab256836) was used. Wild-type and Placental alkaline phosphatase (PLAP) knockout samples were subjected to SDS-PAGE. ab133602 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling Placental alkaline phosphatase (PLAP) with ab133602 at 1/250.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

All lanes : Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602) at 1/10000 dilution

Lane 1 : Human placenta tissue lysate
Lane 2 : Human seminoma tissue lysate
Lane 3 : HeLa cell lysate at 10 µg

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 58 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

ab133602 showing positive staining in Normal placenta tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

Immunohistochemical analysis of paraffin-embedded Human dysgerminoma tissue labelling Placental alkaline phosphatase (PLAP) with ab133602 at 1/250.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

ab133602 showing negative staining in Normal brain tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

ab133602 showing negative staining in Normal liver tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

ab133602 showing positive staining in Seminoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

ab133602 showing negative staining in Normal colon tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

ab133602 showing negative staining in Normal tonsil tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
OI-RD Scanning - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

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Datasheet download

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Publishing research using ab133602? Please let us know so that we can cite the reference in this datasheet.

ab133602 has been referenced in 16 publications.

Herrera-Van Oostdam AS et al. Placental exosomes isolated from urine of patients with gestational diabetes exhibit a differential profile expression of microRNAs across gestation. Int J Mol Med 46:546-560 (2020). PubMed: 32626972
Kohno T et al. Reelin-Nrp1 Interaction Regulates Neocortical Dendrite Development in a Context-Specific Manner. J Neurosci 40:8248-8261 (2020). PubMed: 33009002
Bing T et al. Aptameric Probe Specifically Binding Protein Heterodimer Rather Than Monomers. Adv Sci (Weinh) 6:1900143 (2019). PubMed: 31179220
Xu E et al. Asymmetric expression of GPR126 in the convex/concave side of the spine is associated with spinal skeletal malformation in adolescent idiopathic scoliosis population. Eur Spine J 28:1977-1986 (2019). PubMed: 31079250
Conklin DJ et al. TRPA1 channel contributes to myocardial ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol 316:H889-H899 (2019). PubMed: 30735434

View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - other (Protein lysate from plasma small EVs of pregnant v)

Gel Running Conditions

Reduced Denaturing (4-15%)

Loading amount

20 µg

Specification

Protein lysate from plasma small EVs of pregnant v

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Shuhiba Mohammad

Verified customer

Submitted Aug 25 2021

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (RIPCreZ/HPAP pancreas)

Specification

RIPCreZ/HPAP pancreas

Blocking step

Sniper as blocking agent for 8 minute(s) · Concentration: 100% · Temperature: 21°C

Fixative

Paraformaldehyde

Ms. Christine Beamish

Verified customer

Submitted Sep 17 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

KO cell lines

KO cell lysates

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (16)

Customer reviews and Q&As

Western blot abreview for Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141]

Dissociation constant (K_D)