Recombinant
RabMAb

Recombinant Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (HRP) (ab198554)

Overview

  • Product name

    Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (HRP)
    See all Placental alkaline phosphatase (PLAP) primary antibodies
  • Description

    Rabbit monoclonal [EPR6141] to Placental alkaline phosphatase (PLAP) (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Placental alkaline phosphatase (PLAP) aa 150-250 (extracellular). The exact sequence is proprietary.

  • Positive control

    • WB: Human placenta tissue lysate. IHC-P: Human placenta tissue (normal).
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Dissociation constant (KD)

    KD = 5.10 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin
    Constituents: PBS, 1% BSA, 30% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6141
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab198554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000.
WB 1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 58 kDa).

Target

  • Sequence similarities

    Belongs to the alkaline phosphatase family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alkaline phosphatase antibody
    • Alkaline phosphatase placental antibody
    • Alkaline phosphatase placental type antibody
    • Alkaline phosphatase Regan isozyme antibody
    • ALP antibody
    • Alp1 antibody
    • ALPP antibody
    • FLJ61142 antibody
    • Germ-cell alkaline phosphatase antibody
    • nagao isozyme antibody
    • OTTHUMP00000164354 antibody
    • PALP antibody
    • Placental alkaline phosphatase 1 antibody
    • placental heat-stable alkaline phosphatase antibody
    • placental type antibody
    • PLAP antibody
    • PLAP-1 antibody
    • PLAP1 antibody
    • PPB1_HUMAN antibody
    see all

Images

  • Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (HRP) (ab198554) at 1/5000 dilution + Human placenta tissue lysate - total protein (ab29745) at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab198554 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • IHC image of Placental alkaline phosphatase (PLAP) staining in a section of formalin-fixed paraffin-embedded normal human placenta*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab198554 at 1/1000 dilution for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

ab198554 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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