Recombinant
RabMAb

Recombinant Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)

Overview

  • Product name

    Anti-Placental alkaline phosphatase (PLAP) antibody [SP15]
    See all Placental alkaline phosphatase (PLAP) primary antibodies
  • Description

    Rabbit monoclonal [SP15] to Placental alkaline phosphatase (PLAP)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Epitope

    Not determined
  • Positive control

    • Seminoma and placenta FC: HeLa cells
  • General notes

    This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Primary antibody notes

    This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).
  • Clonality

    Monoclonal
  • Clone number

    SP15
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/200.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

WB Use at an assay dependent concentration.

Target

  • Sequence similarities

    Belongs to the alkaline phosphatase family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alkaline phosphatase antibody
    • Alkaline phosphatase placental antibody
    • Alkaline phosphatase placental type antibody
    • Alkaline phosphatase Regan isozyme antibody
    • ALP antibody
    • Alp1 antibody
    • ALPP antibody
    • FLJ61142 antibody
    • Germ-cell alkaline phosphatase antibody
    • nagao isozyme antibody
    • OTTHUMP00000164354 antibody
    • PALP antibody
    • Placental alkaline phosphatase 1 antibody
    • placental heat-stable alkaline phosphatase antibody
    • placental type antibody
    • PLAP antibody
    • PLAP-1 antibody
    • PLAP1 antibody
    • PPB1_HUMAN antibody
    see all

Images

  • Flow cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Placental alkaline phosphatase (PLAP) with purified ab16695 at 1/200 dilution (0.6 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.
  • Human seminoma stained with ab16695.
  • Immunohistochemical analysis of paraffin-embedded human seminoma tissue labeling Alkaline Phosphatase with ab137879 at 1/50 dilution.

References

This product has been referenced in:

See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry.

Here are the answers to your questions:

1. ab16517 - immunogen is Full length native protein (purified) (Human) and epitope has not been mapped
2. ab16695 - immunogen is Full length native protein (purified) (Human) and epitope has not been mapped
3. ab70988 - peptide between 39˜69 amino acids from the N-terminal region of human BMPR1A

I hope this is helpful. Please contact me again if you have any further questions.

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Question
Answer

Please see the western blot in following reference noted under the 'References' section of the online datasheet for ab16695: Taylor DD et al. Pregnancy-associated exosomes and their modulation of T cell signaling. J Immunol 176:1534-42 (2006). WB; Human. PubMed: 16424182 I hope this is helpful. Please contact us again if you have any further questions.

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