Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP15] to Placental alkaline phosphatase (PLAP) - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Mouse, Human
Product nameAnti-Placental alkaline phosphatase (PLAP) antibody [SP15] - BSA and Azide free
See all Placental alkaline phosphatase (PLAP) primary antibodies
DescriptionRabbit monoclonal [SP15] to Placental alkaline phosphatase (PLAP) - BSA and Azide free
Tested applicationsSuitable for: IHC-P, Flow Cytmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Recombinant full length protein within Human Placental alkaline phosphatase (PLAP). The exact sequence is proprietary.
- IHC-P: Seminoma and placenta tissues. Flow Cyt: HeLa cells
FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.
ab218848 is the carrier-free version of ab16695 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab218848 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
Our Abpromise guarantee covers the use of ab218848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
|Flow Cyt||Use at an assay dependent concentration.|
Sequence similaritiesBelongs to the alkaline phosphatase family.
Cellular localizationCell membrane.
- Information by UniProt
- Alkaline phosphatase antibody
- Alkaline phosphatase placental antibody
- Alkaline phosphatase placental type antibody
Flow cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Placental alkaline phosphatase (PLAP) with purified ab16695 at 1/200 dilution (0.6 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.
This data was developed using the same antibody clone in a different buffer formulation containing Tris buffered saline, BSA and sodium azide (ab16695).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab218848 has not yet been referenced specifically in any publications.