Recombinant
RabMAb

Anti-Placental Protein 14 / Glycodelin A antibody [EP870Y] (ab53289)

Overview

  • Product name
    Anti-Placental Protein 14 / Glycodelin A antibody [EP870Y]
    See all Placental Protein 14 / Glycodelin A primary antibodies
  • Description
    Rabbit monoclonal [EP870Y] to Placental Protein 14 / Glycodelin A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Placental Protein 14/ Glycodelin A aa 150-250 (C terminal). The exact sequence is proprietary.
    Database link: P09466

  • Positive control
    • WB: TPA-treated K562 cell lysate. Human placenta, kidney, and brain tissue lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EP870Y
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

Target

  • Function
    This protein is, quantitatively, the main protein synthesized and secreted in the endometrium from mid-luteal phase of the menstrual cycle and during the first semester of pregnancy.
  • Sequence similarities
    Belongs to the calycin superfamily. Lipocalin family.
  • Post-translational
    modifications
    At least two differentially glycosylated forms are found. Glycodelin-A (GdA) (amniotic fluid) with contraceptive and immunosuppressive activities and glycodelin-S (Gds) (seminal plasma) whose role is not yet known. The gender-specific glycosylation may serve to regulate key process involved in human reproduction.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha uterine protein antibody
    • gD antibody
    • GdA antibody
    • GdF antibody
    • GdS antibody
    • Glycodelin A antibody
    • Glycodelin antibody
    • Glycodelin F antibody
    • Glycodelin S antibody
    • MGC138509 antibody
    • MGC142288 antibody
    • PAEG antibody
    • PAEP antibody
    • PAEP_HUMAN antibody
    • PEG antibody
    • PEP antibody
    • Placental protein 14 antibody
    • PP14 antibody
    • Pregnancy-associated endometrial alpha-2 globulin antibody
    • Progestagen-associated endometrial protein antibody
    • Progesterone-associated endometrial protein antibody
    see all

Images

  • All lanes : Anti-Placental Protein 14 / Glycodelin A antibody [EP870Y] (ab53289) at 0.3 µg/ml (purified)

    Lane 1 : Human placenta lysates
    Lane 2 : Human kidney lysates
    Lane 3 : Human brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 21 kDa
    Observed band size: 24 kDa (why is the actual band size different from the predicted?)



    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-Placental Protein 14 / Glycodelin A antibody [EP870Y] (ab53289) at 1/500 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
    Lane 2 : TPA-treated K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

References

This product has been referenced in:
  • Turco MY  et al. Long-term, hormone-responsive organoid cultures of human endometrium in a chemically defined medium. Nat Cell Biol 19:568-577 (2017). WB ; Human . Read more (PubMed: 28394884) »
  • Uysal S  et al. Correlation of endometrial glycodelin expression and pregnancy outcome in cases with polycystic ovary syndrome treated with clomiphene citrate plus metformin: a controlled study. Obstet Gynecol Int 2015:278591 (2015). Read more (PubMed: 25815012) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human endometrium cells, RL95-2)
Permeabilization
No
Specification
human endometrium cells, RL95-2
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Username

Miss. Tatyana Kuperman

Verified customer

Submitted Sep 05 2018

Thank you for contacting us. Unfortunately the data stated on this datasheet was unfortunately entered in error. This antibody does not react with mouse or rat Placental Protein 14. This error has now been remedied. Thank you for bringing this to our a...

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Thank you for your enquiry. At this time we know of no references which have used ab53289. Any published papers we are aware of that use ab53289 would be listed under the 'References' tab at the top of the online datasheet. I hope this he...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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