Anti-PLAGL1 / ZAC antibody [8D8C5] (ab181457)
Key features and details
- Mouse monoclonal [8D8C5] to PLAGL1 / ZAC
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PLAGL1 / ZAC antibody [8D8C5]
See all PLAGL1 / ZAC primary antibodies -
Description
Mouse monoclonal [8D8C5] to PLAGL1 / ZAC -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Pig -
Immunogen
Recombinant fragment corresponding to Human PLAGL1/ ZAC aa 100-250. (Expressed in E.coli).
Database link: Q9UM63 -
Positive control
- Human PLAGL1 / ZAC recombinant protein; PLAGL1 / ZAC (aa: 118-222)-hIgGFc transfected HEK293 cell lysate; Human ovarian cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Contains 0.5% protein stabilizer (Amino acid =85%, pH(1% water solution) =7.0~7.5, Water =2%, As(mg/kg) =0.5, Pb(mg/kg) =0.1) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
8D8C5 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 51 kDa.
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IHC-P |
1/200 - 1/1000.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 51 kDa. |
IHC-P
1/200 - 1/1000. |
Target
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Function
Shows weak transcriptional activatory activity. Transcriptional regulator of the type 1 receptor for pituitary adenylate cyclase-activating polypeptide. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 7 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5325 Human
- Entrez Gene: 733596 Pig
- Omim: 603044 Human
- SwissProt: Q9UM63 Human
- SwissProt: Q2I689 Pig
- Unigene: 725973 Human
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Alternative names
- DKFZp781P1017 antibody
- Lost on transformation 1 antibody
- Lost on Transformation antibody
see all
Images
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All lanes : Anti-PLAGL1 / ZAC antibody [8D8C5] (ab181457) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : PLAGL1 / ZAC (aa: 118-222)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 51 kDa -
Anti-PLAGL1 / ZAC antibody [8D8C5] (ab181457) at 1/500 dilution + Human PLAGL1 / ZAC recombinant protein
Predicted band size: 51 kDaExpected MWt is 37.5 kDa.
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Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling PLAGL1 / ZAC with ab181457 at 1/200 dilution, with DAB staining.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab181457 has not yet been referenced specifically in any publications.