Product nameAnti-PLAGL1 / ZAC antibody [EPR7523]
See all PLAGL1 / ZAC primary antibodies
DescriptionRabbit monoclonal [EPR7523] to PLAGL1 / ZAC
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human PLAGL1/ ZAC aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
- Human fetal liver and Human fetal kidney, 293T, HepG2 and SH-SY5Y lysates. Permeabilized HepG2 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.71 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129063 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionShows weak transcriptional activatory activity. Transcriptional regulator of the type 1 receptor for pituitary adenylate cyclase-activating polypeptide.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 7 C2H2-type zinc fingers.
- Information by UniProt
- DKFZp781P1017 antibody
- Lost on transformation 1 antibody
- Lost on Transformation antibody
All lanes : Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063) at 1/10000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : 293T cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
ab129063 at 1/10 dilution staining PLAGL1 / ZAC in permeabilized HepG2 cells by Flow Cytometry (red) or a rabbit IgG negative (green).
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Chu YW et al. The cytotoxic mechanism of epigallocatechin gallate on proliferative HaCaT keratinocytes. J Biomed Sci 24:55 (2017). Read more (PubMed: 28810862) »
- Godlewski J et al. PLAGL1 (ZAC1/LOT1) Expression in Clear Cell Renal Cell Carcinoma: Correlations with Disease Progression and Unfavorable Prognosis. Anticancer Res 36:617-24 (2016). Read more (PubMed: 26851016) »