Recombinant Anti-PLAGL1 / ZAC antibody [EPR7523] - BSA and Azide free (ab248273)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7523] to PLAGL1 / ZAC - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-PLAGL1 / ZAC antibody [EPR7523] - BSA and Azide free
See all PLAGL1 / ZAC primary antibodies -
Description
Rabbit monoclonal [EPR7523] to PLAGL1 / ZAC - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
Application Species Flow Cyt HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248273 is the carrier-free version of ab129063 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248273 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 1.71 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR7523 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Human
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WB |
Human
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Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
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Notes |
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Flow Cyt
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
Target
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Function
Shows weak transcriptional activatory activity. Transcriptional regulator of the type 1 receptor for pituitary adenylate cyclase-activating polypeptide. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 7 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5325 Human
- Omim: 603044 Human
- SwissProt: Q9UM63 Human
- Unigene: 725973 Human
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Alternative names
- DKFZp781P1017 antibody
- Lost on transformation 1 antibody
- Lost on Transformation antibody
see all
Images
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All lanes : Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063) at 1/10000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDaThis data was developed using ab129063, the same antibody clone in a different buffer formulation.
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This data was developed using ab129063, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab248273 has not yet been referenced specifically in any publications.