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RecombinantRabMAb

Recombinant Anti-PLAGL1 / ZAC antibody [EPR7523] - BSA and Azide free (ab248273)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - Anti-PLAGL1 / ZAC antibody [EPR7523] - BSA and Azide free (ab248273)
  • Flow Cytometry - Anti-PLAGL1 / ZAC antibody [EPR7523] - BSA and Azide free (ab248273)
  • OI-RD Scanning - Anti-PLAGL1 / ZAC antibody [EPR7523] - BSA and Azide free (ab248273)
  • Anti-PLAGL1 / ZAC antibody [EPR7523] - BSA and Azide free (ab248273)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7523] to PLAGL1 / ZAC - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human

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Conjugation
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PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)

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Overview

  • Product name

    Anti-PLAGL1 / ZAC antibody [EPR7523] - BSA and Azide free
    See all PLAGL1 / ZAC primary antibodies
  • Description

    Rabbit monoclonal [EPR7523] to PLAGL1 / ZAC - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248273 is the carrier-free version of ab129063 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab248273 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 1.71 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7523
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Stem Cells
    • Neural Stem Cells
    • Intracellular

Associated products

  • Alternative Versions

    • Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • Hep G2 nuclear extract lysate (ab14660)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
WB
Human
Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
Notes
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
  • Application notes
    Is unsuitable for ICC,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Shows weak transcriptional activatory activity. Transcriptional regulator of the type 1 receptor for pituitary adenylate cyclase-activating polypeptide.
    • Sequence similarities

      Belongs to the krueppel C2H2-type zinc-finger protein family.
      Contains 7 C2H2-type zinc fingers.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q9UM63 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5325 Human
      • Omim: 603044 Human
      • SwissProt: Q9UM63 Human
      • Unigene: 725973 Human
      • Alternative names

        • DKFZp781P1017 antibody
        • Lost on transformation 1 antibody
        • Lost on Transformation antibody
        • LOT-1 antibody
        • LOT1 antibody
        • MGC126275 antibody
        • MGC126276 antibody
        • PLAG like 1 antibody
        • PLAGL1 antibody
        • PLAL1_HUMAN antibody
        • Pleiomorphic adenoma gene like 1 antibody
        • Pleiomorphic adenoma gene like protein 1 antibody
        • Pleiomorphic adenoma-like protein 1 antibody
        • Tumor supressor ZAC antibody
        • ZAC antibody
        • ZAC tumor supressor antibody
        • ZAC1 antibody
        • Zinc finger protein PLAGL1 antibody
        see all

      Images

      • Western blot - Anti-PLAGL1 / ZAC antibody [EPR7523] - BSA and Azide free (ab248273)
        Western blot - Anti-PLAGL1 / ZAC antibody [EPR7523] - BSA and Azide free (ab248273)
        All lanes : Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063) at 1/10000 dilution

        Lane 1 : Human fetal liver tissue lysate
        Lane 2 : Human fetal kidney tissue lysate
        Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
        Lane 4 : HepG2 cell lysate
        Lane 5 : SH-SY5Y cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 51 kDa
        Observed band size: 51 kDa



        This data was developed using ab129063, the same antibody clone in a different buffer formulation.

      • Flow Cytometry - Anti-PLAGL1 / ZAC antibody [EPR7523] - BSA and Azide free (ab248273)
        Flow Cytometry - Anti-PLAGL1 / ZAC antibody [EPR7523] - BSA and Azide free (ab248273)
        This data was developed using ab129063, the same antibody clone in a different buffer formulation.ab129063 at 1/10 dilution staining PLAGL1 / ZAC in permeabilized HepG2 cells by Flow Cytometry (red) or a rabbit IgG negative (green).
      • OI-RD Scanning - Anti-PLAGL1 / ZAC antibody [EPR7523] - BSA and Azide free (ab248273)
        OI-RD Scanning - Anti-PLAGL1 / ZAC antibody [EPR7523] - BSA and Azide free (ab248273)
        This data was developed using ab129063, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-PLAGL1 / ZAC antibody [EPR7523] - BSA and Azide free (ab248273)
        Anti-PLAGL1 / ZAC antibody [EPR7523] - BSA and Azide free (ab248273)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

      References (0)

      Publishing research using ab248273? Please let us know so that we can cite the reference in this datasheet.

      ab248273 has not yet been referenced specifically in any publications.

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