Recombinant Anti-Plakophilin 1 antibody [EPR14890] (ab183512)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14890] to Plakophilin 1
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Plakophilin 1 antibody [EPR14890]
See all Plakophilin 1 primary antibodies -
Description
Rabbit monoclonal [EPR14890] to Plakophilin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Mouse skin, Human skin and Human tonsil tissue lysates and A431 and HACAT cell lysates; HACAT cells
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14890 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/20000. Detects a band of approximately 75 kDa (predicted molecular weight: 81 kDa).
|
|
ICC/IF |
1/100.
|
|
Flow Cyt (Intra) |
1/90.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
---|
WB
1/1000 - 1/20000. Detects a band of approximately 75 kDa (predicted molecular weight: 81 kDa). |
ICC/IF
1/100. |
Flow Cyt (Intra)
1/90. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Seems to play a role in junctional plaques. Contributes to epidermal morphogenesis. -
Tissue specificity
Isoform 2 is widely expressed. Isoform 1 is expressed in stratified squamous, complex, glandular duct and bladder epithelia. -
Involvement in disease
Ectodermal dysplasia-skin fragility syndrome -
Sequence similarities
Belongs to the beta-catenin family.
Contains 9 ARM repeats. -
Cellular localization
Nucleus and Nucleus. Cell junction, desmosome. - Information by UniProt
-
Database links
- Entrez Gene: 5317 Human
- Entrez Gene: 18772 Mouse
- Omim: 601975 Human
- SwissProt: Q13835 Human
- SwissProt: P97350 Mouse
- Unigene: 497350 Human
- Unigene: 4494 Mouse
-
Alternative names
- B6P antibody
- Band 6 protein antibody
- PKP1 antibody
see all
Images
-
All lanes : Anti-Plakophilin 1 antibody [EPR14890] (ab183512) at 1/20000 dilution
Lane 1 : Mouse skin tissue lysate
Lane 2 : A431 cell lysate
Lane 3 : HACAT cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 81 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
All lanes : Anti-Plakophilin 1 antibody [EPR14890] (ab183512) at 1/5000 dilution
Lane 1 : Human skin tissue lysate
Lane 2 : Human tonsil tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 81 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
Immunofluorescent staining of 4% paraformaldehyde fixed HACAT cells labeling Plakophilin 1 using ab183512 at a 1/100 dilution followed by Goat anti Rabbit IgG (Alexa Fluor®488) secondary at 1/200 dilution. Counterstained with Dapi (bue).
-
Intracellular flow cytometric analysis of HACAT cells (2% paraformaldehyde fixed) labeling Plakophilin 1 with ab183512 at 1/90 dilution (red) compared to a Rabbit monoclonal isotype control (green).
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (2)
ab183512 has been referenced in 2 publications.
- Suga H et al. Keratinocyte Proline-Rich Protein Deficiency in Atopic Dermatitis Leads to Barrier Disruption. J Invest Dermatol 139:1867-1875.e7 (2019). PubMed: 30905808
- Wang F et al. Keratin 6 regulates collective keratinocyte migration by altering cell-cell and cell-matrix adhesion. J Cell Biol 217:4314-4330 (2018). PubMed: 30389720