Anti-Plakophilin 2/PKP2 antibody [8H6] (ab151402)
![Flow Cytometry - Anti-Plakophilin 2/PKP2 antibody [8H6] (ab151402)](https://www.abcam.com/ps/products/151/ab151402/Images/ab151402-210388-anti-plakophilin-2-antibody-8h6-flow-cytometry.jpg "Flow Cytometry - Anti-Plakophilin 2/PKP2 antibody [8H6] (ab151402)")
Key features and details
- Mouse monoclonal [8H6] to Plakophilin 2/PKP2
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Plakophilin 2/PKP2 antibody [8H6]
See all Plakophilin 2/PKP2 primary antibodies -
Description
Mouse monoclonal [8H6] to Plakophilin 2/PKP2 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Plakophilin 2/PKP2 aa 1-350.
Database link: Q99959 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
8H6 -
Myeloma
NS1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/100. Predicted molecular weight: 97 kDa.
|
| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
1/100. Predicted molecular weight: 97 kDa. |
|
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
May play a role in junctional plaques. -
Tissue specificity
Detected in heart right ventricle (at protein level). Widely expressed. Found at desmosomal plaques in simple and stratified epithelia and in non-epithelial tissues such as myocardium and lymph node follicles. In most stratified epithelia found in the desmosomes of the basal cell layer and seems to be absent from suprabasal strata. -
Involvement in disease
Familial arrhythmogenic right ventricular dysplasia 9 -
Sequence similarities
Belongs to the beta-catenin family.
Contains 8 ARM repeats. -
Cellular localization
Nucleus. Cell junction > desmosome. Nuclear and associated with desmosomes. - Information by UniProt
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Database links
- Entrez Gene: 5318 Human
- Omim: 602861 Human
- SwissProt: Q99959 Human
- Unigene: 164384 Human
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Alternative names
- ARVD 9 antibody
- ARVD9 antibody
- PKP 2 antibody
see all
Images
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Flow Cytometry - Anti-Plakophilin 2/PKP2 antibody [8H6] (ab151402)Overlay histogram showing HeLa cells stained with ab151402 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab151402, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Western blot - Anti-Plakophilin 2/PKP2 antibody [8H6] (ab151402)](https://www.abcam.com/ps/products/151/ab151402/Images/ab151402-158271-anti-plakophilin-2-antibody-8h6-western-blot.jpg)
Western blot - Anti-Plakophilin 2/PKP2 antibody [8H6] (ab151402)Anti-Plakophilin 2/PKP2 antibody [8H6] (ab151402) at 1/100 dilution + HaCat Human keratinocyte cells
Predicted band size: 97 kDa
Observed band size: 92 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab151402 has been referenced in 1 publication.
- Wang L et al. Arrhythmogenic cardiomyopathy: Identification of desmosomal gene variations and desmosomal protein expression in variation carriers. Exp Ther Med 15:2255-2262 (2018). PubMed: 29456632