Overview

  • Product name

    Anti-Plakophilin 3 antibody [EPR5560]
    See all Plakophilin 3 primary antibodies
  • Description

    Rabbit monoclonal [EPR5560] to Plakophilin 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Plakophilin 3 aa 200-300. The exact sequence is proprietary.

  • Positive control

    • Caco2 and HACAT cell lysates; Human breast and kidney tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    Images

    • All lanes : Anti-Plakophilin 3 antibody [EPR5560] (ab109441) at 1/10000 dilution

      Lane 1 : Caco2 cell lysate
      Lane 2 : HACAT cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 87 kDa
      Observed band size: 87 kDa

    • ab109441 at 1/100 dilution staining Human paraffin-embedded breast tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • ab109441 at 1/100 dilution staining paraffin-embedded Human kidney tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Zhou L  et al. Mice with Hepatic Loss of the Desmosomal Protein ?-Catenin Are Prone to Cholestatic Injury and Chemical Carcinogenesis. Am J Pathol 185:3274-89 (2015). Read more (PubMed: 26485505) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for contacting us.

    We would not recommend to freeze the antibody again once it has been thawed and used on a few samples. These repeated freeze/thaw cycles could damage the antibody. Instead, we would suggest to prepare and freeze small aliquots of the antibody. In case you have already frozen the whole vial before aliquotting, it should be alright to defrost it, prepare the aliquots and freeze them once again. Just keep in mind that any reduced signal might be caused by this.

    Once the antibody (an aliquot) has been defrosted it can be kept at 4ºC for a few weeks. Here, we would recommend to store the stock solution and preparedilutions freshly when required.

    Please find enclosed our antibody storage guide for further reference.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Answer

    Thank you for your enquiry.

    I can confirm that ab109441 has been tested successfully in IHC-P at a dilution of 1/100 - 1/250, as stated on the datasheet. Please note this is a guideline only, and may require some further optimization.

    For a small section of tissue, you can pipette 50 ul of diluted antibody solution ontoone slide.For larger sections, you may require 100 - 200 ul per slide. As long as thesolution covers the section and does not dry out during incubation.

    Unfortunately, I am sorry this antibody is currently out of stock. The expected wait time is 2 - 3 weeks to delivery. This information can also be found on the datasheet. I can suggest to select the correct country in the top right hand corner of the screen to see the correct information:

    https://www.abcam.com/index.html?datasheet=109441 (or use the following: https://www.abcam.com/index.html?datasheet=109441).

    I hope this will be helpful. If you have any further questions, please do not hesitate to contact me.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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