Recombinant Anti-Plakophilin 3 antibody [EPR5560] (ab109441)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5560] to Plakophilin 3
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Plakophilin 3 antibody [EPR5560]
See all Plakophilin 3 primary antibodies -
Description
Rabbit monoclonal [EPR5560] to Plakophilin 3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Caco2 and HACAT cell lysates; Human breast and kidney tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.66 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5560 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Target
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Function
May play a role in junctional plaques. -
Tissue specificity
Found in desmosomes of most simple and stratified epithelia. Not found in foreskin fibroblasts and various sarcoma-derived cell lines. Beside dendritic reticular cells of lymphatic follicles not found in non-epithelial desmosome-bearing tissues. -
Sequence similarities
Belongs to the beta-catenin family.
Contains 8 ARM repeats. -
Cellular localization
Nucleus. Cell junction > desmosome. Nuclear and associated with desmosomes. - Information by UniProt
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Database links
- Entrez Gene: 11187 Human
- Entrez Gene: 56460 Mouse
- Entrez Gene: 293619 Rat
- Omim: 605561 Human
- SwissProt: Q9Y446 Human
- SwissProt: Q9QY23 Mouse
- Unigene: 534395 Human
- Unigene: 350037 Mouse
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Alternative names
- PKP 3 antibody
- PKP3 antibody
- PKP3_HUMAN antibody
see all
Images
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All lanes : Anti-Plakophilin 3 antibody [EPR5560] (ab109441) at 1/10000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PKP3 CRISPR-Cas9 edited HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 87 kDa
Observed band size: 70, 80 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-Plakophilin 3 antibody [EPR5560] staining at 1/10000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab109441 was shown to bind specifically to Plakophilin 3. A band was observed at 70 and 80 kDa in wild-type HeLa cell lysates with no signal observed at this size in PKP3 CRISPR-Cas9 edited cell line ab265539 (CRISPR-Cas9 edited cell lysate ab258120). The band observed in the CRISPR-Cas9 edited lysate lane below 70 and 80 kDa is likely to represent a truncated form of Plakophilin 3. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and PKP3 CRISPR-Cas9 edited HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes : Anti-Plakophilin 3 antibody [EPR5560] (ab109441) at 1/10000 dilution
Lane 1 : Caco-2 cell lysate
Lane 2 : HACAT cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 87 kDa
Observed band size: 87 kDa -
ab109441 at 1/100 dilution staining Human paraffin-embedded breast tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab109441 at 1/100 dilution staining paraffin-embedded Human kidney tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab109441 has been referenced in 2 publications.
- Raghavan R et al. Plakophilin3 loss leads to increased adenoma formation and rectal prolapse in APCmin mice. Biochem Biophys Res Commun 586:14-19 (2022). PubMed: 34823217
- Zhou L et al. Mice with Hepatic Loss of the Desmosomal Protein ?-Catenin Are Prone to Cholestatic Injury and Chemical Carcinogenesis. Am J Pathol 185:3274-89 (2015). PubMed: 26485505