Overview

  • Product name

    Plant Chromatin Extraction Kit
    See all Chromatin Extraction kits
  • Sample type

    Tissue
  • Assay time

    2h 00m
  • Species reactivity

    Reacts with: Plants
  • Product overview

    Abcam's Plant Chromatin Extraction Kit (ab156906) is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complexes from plants in a simple and rapid format. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays, or nuclear enzyme assays. It is also the recommended method for obtaining chromatin required by Abcam’s one-hour ChIP method using the ChIP Kit - Plants (ab117137).

  • Notes

    Chromatin immunoprecipitation (ChIP) offers an advantageous tool for studying protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the specific sequences of a gene in living cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) techniques. For example, the measurement of the amount of methylated histone H3 at lysine 9 (H3K9me) associated with a specific gene promoter region under various conditions can be achieved through a ChIP-PCR assay, while recruitment of H3K9me to the promoters on a genome-wide scale can be detected by ChIP-chip. In particular, a ChIP method with specific antibodies directly against various transcriptional factors is widely in demand.

Properties

Images

  • ChIP analysis of tri-methyl H3K27 enriched in AGAMOUS gene with chromatin extract prepared from 2-week-old icu2-1/icu2-1 seedlings using Abcam's Plant Chromatin Extraction Kit (ab156906).

Protocols

References

This product has been referenced in:

  • Capote T  et al. ChIP-Seq reveals that QsMYB1 directly targets genes involved in lignin and suberin biosynthesis pathways in cork oak (Quercus suber). BMC Plant Biol 18:198 (2018). Read more (PubMed: 30223777) »
See 1 Publication for this product

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