Overview

  • Product name

    Plant tissue extraction kit
  • Product overview

    Abcam’s Plant tissue extraction kit (ab206999) enables effective extraction of soluble protein and metabolites from a range of dry and fresh plant tissues without the use of liquid nitrogen. The extracted proteins and metabolites can be used in applications such as western blotting, 2-D gel electrophoresis, immunoprecipitation assay, enzymatic activity assays, quantitation of metabolites etc.


    The kit includes optimized Extraction Buffer to extract plant protein and metabolites, Protease Inhibitor Cocktail to prevent protein degradation and Homogenization Pestles and Tubes to homogenize the plant tissue. This easy-to-use and rapid procedure ensures consistent protein yield (~ 2-9 mg/mL, depending on the sample type).

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 50 tests
    Homogenization Pestles 3 units
    Homogenization Tubes 3 x 1.5ml
    Plant Extraction Buffer 1 x 25ml
    Protease Inhibitor Cocktail (200X) 1 x 1ml

Images

  • SDS-PAGE of plant tissue lysates prepared using ab206999: 25 μL of lysate from lettuce (20  μg, lane 2), green pepper (20 μg, lane 3), avocado (40  μg, lane 4) and orchid leaf (10 μg, lane 5) were loaded onto a 4-20% SDS-PAGE and, following electrophoresis, stained with Coomassie blue. Lane 1: Marker.

  • Measurement of Glucose-6-Phosphate in orchid leaf lysate (5 μg) using a G6P Fluorometric Assay Kit. The lysate was prepared using ab206999.

  • Measurement of glucose-6-phosphate dehydrogenase activity in orchid leaf lysate (0.6 μg) using a G6PDH Activity Assay Kit. The lysate was prepared using ab206999.

Protocols

References

ab206999 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab206999.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up