Product nameAnti-Plasma Kallikrein 1B antibody [13G11]
See all Plasma Kallikrein 1B primary antibodies
DescriptionMouse monoclonal [13G11] to Plasma Kallikrein 1B
Tested applicationsSuitable for: IHC-P, ELISA, Inhibition Assay, WBmore details
Species reactivityReacts with: Rat, Human, Chimpanzee, Baboon, Rhesus monkey
Full length native prekallikrein protein (purified) (Human)
EpitopeThe specific epitope, found on the prekallikrein / kallikrein heavy chain is involved in the interaction between prekallikrein and factor XIIa.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPBS, pH7.2
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab1006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ELISA||Use at an assay dependent concentration.|
|Inhibition Assay||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.
Dilute in PBS or medium which is identical to that used in the assay system. This antibody inhibits activation of prekallikrein by approximately 60-80%.
FunctionThe enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
Involvement in diseaseDefects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect.
Sequence similaritiesBelongs to the peptidase S1 family. Plasma kallikrein subfamily.
Contains 4 apple domains.
Contains 1 peptidase S1 domain.
- Information by UniProt
- Fletcher factor antibody
- kallikrein B plasma antibody
- Kallikrein B plasma (Fletcher factor) 1 antibody
ab1006 staining Plasma Kallikrein 1B by Western Blot.
Lanes 1-6: Human Vitreous (4ug/lane)
Lane 7: PK
Lane 8: PK + Factor XII + HK
An HRP conjugated Goat anti-mouse antibody was used as the secondary.
The antibody was used to block the conversion of prekallikrein to kallikrein in vivo and in vitro (PMID: 17259996). The antibody also reacts with rat retina and vitreous.
ab1006 (4µg/ml) staining plasma kallikrein in human liver, using an automated system (DAKO Autostainer Plus). Using this protocol there is weak cytoplasmic staining of a subpopulation of hepatocytes.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
This product has been referenced in:
- Badawy AA et al. Expression of Epidermal Growth Factor Receptor and Transforming Growth Factor Alpha in Cancer Bladder: Schistosomal and Non-Schistosomal. Curr Urol 9:192-201 (2017). Read more (PubMed: 28413380) »
- Liu J et al. Hyperglycemia-induced cerebral hematoma expansion is mediated by plasma kallikrein. Nat Med 17:206-10 (2011). Functional Studies ; Rat . Read more (PubMed: 21258336) »