Overview

  • Product name
    Anti-Plasma Kallikrein 1B antibody
    See all Plasma Kallikrein 1B primary antibodies
  • Description
    Rabbit polyclonal to Plasma Kallikrein 1B
  • Host species
    Rabbit
  • Specificity
    This antibody is specific for Plasma Kallikrein 1B (amino end of the catalytic domain).
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide based on the amino end of the catalytic domain of human Plasma Kallikrein 1B.

    Read Abcam's proprietary immunogen policy (Peptide available as ab43118.)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituent: 50% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab44392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 71 kDa. 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.

Target

  • Function
    The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
  • Involvement in disease
    Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect.
  • Sequence similarities
    Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
    Contains 4 apple domains.
    Contains 1 peptidase S1 domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fletcher factor antibody
    • kallikrein B plasma antibody
    • Kallikrein B plasma (Fletcher factor) 1 antibody
    • Kallikrein B, plasma 1 antibody
    • Kallikrein B1 antibody
    • Kininogenin antibody
    • KLK3 antibody
    • KLKB1 antibody
    • KLKB1_HUMAN antibody
    • PKK antibody
    • PKKD antibody
    • Plasma kallikrein antibody
    • Plasma kallikrein B1 antibody
    • Plasma kallikrein light chain antibody
    • Plasma prekallikrein antibody
    • PPK antibody
    • Prekallikrein antibody
    see all

Images

  • ab44392 staining Kallikrein 1B in human temporal neocortical tissue sections IHC-P . Tissue samples were fixed with formaldehyde and blocked with 10% serum for 20 minutes at 23°C: antigen retrieval was performed by heat mediation in citrate buffer.. Samples were incubated with primary antibody (1/100) for 18 hours at 23°C . A biotin-conjugated horse anti Rabbit IgG  polyclonal (1/50) was used as secondary antibody.

    See Abreview

References

ab44392 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Purified protein (Plasma)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE gel)
Loading amount
2 µg
Specification
Plasma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

匡 勅使川原

Verified customer

Submitted Jun 19 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Pig Purified protein (porcine plasma, porcine articular capsule extract)
Gel Running Conditions
Non-reduced Denaturing (10% PAGE)
Loading amount
0.2 µg
Specification
porcine plasma, porcine articular capsule extract
Blocking step
Tween 20 as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Feb 10 2016

Answer

I will send the alignment results for ab116517, hopefully later today.

I am glad ab1006 is working, at least with human samples. If you have time, we encourage our customers to send us reviews of their results, positive or negative, via our Abreview program. (The ab1006 datasheet has several examples). In return, you receive credits towards future purchases or Amazon gift cards. Details are at the following URL:

www.abcam.com/abreviews

Read More

Answer

This is frustrating for us too, finding incorrect information on our datasheets. We try to minimize it but there is clearly work to be done.

I did not realize you had already purchased the antibody. If you would still like to try it with rat samples and it fails, I will offer a refund or credit or replacement with another antibody. Otherwise, I can send you instructions on how to return it for a full refund.

Of the other KLK1B antibodies, ab43084 and ab44203 have similar differences with the rat protein (8/35 and 10/35 residue mismatches respectively). It is unlikely these have been tested with rat tissues as they are from the same laboratory that provided ab44392. I am confirming with that lab.

The other two candidates are ab116517 and ab75228 but the latter appears to be detecting the heavy chain (as does ab1006). I will have the originating laboratory align the ab116517 immunogen with the rat KLK1B sequence, and will confirm if it is on the light chain.

Antibody ab1006 reactivity with rat was not confirmed in-house but was added to the datasheet based on the second reference listed on the datasheet, which shows a blot in figure 5A.

Morris KE et al. Autonomic nervous system regulates secretion of anti-inflammatory prohormone SMR1 from rat salivary glands. Am J Physiol Cell Physiol 296:C514-24 (2009). PubMed: 19109528

Read More

Answer

My apologies: the laboratory that supplies this to us confirmed that they have not tested this for reactivity to rat protein, for have we or, to our knowledge, any of our customers. It is not clear why our datasheet lists rat in the "Reacts With" field but I am assuming there was a miscommunication.

I am going to remove rat reactivity from the datasheet, so it will not be covered by our guarantee. If you are still interested in trying it, we do offer a free antibody in return for a review of an antibody tested in previously untested species or application.

In brief, you would buy ab44392 at full price, test it against your rat samples in one of the listed tested applications (ELISA, IHC-P, WB), and send us a review via our "Abreview" program. (Details can be found at www.abcam.com/abreviews. It is essentially a paragraph description of your protocol and results).

At that point, a code that is issued to you becomes active and can be used (within four months of the purchase of ab44392) to obtain a free antibody.

Please let me know if you are interested.

Read More

Answer

Thank you for contacting us. Unfortunately, the immunogen sequence for that antibody is in fact proprietary, and we do not offer or accept confidentiality agreements, for this or other immunogen sequences.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Cell (human brain)
Specification
human brain
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 0.5% · Temperature: 22°C
Type
Sandwich (Detection)

Miss. Emma Ashby

Verified customer

Submitted Dec 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (temporal neocortex)
Specification
temporal neocortex
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Nov 26 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Purified protein (human plasma)
Loading amount
4 µg
Specification
human plasma
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Oct 26 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (blood)
Loading amount
20 µg
Specification
blood
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 16 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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