Anti-Plasma Kallikrein 1B antibody (ab44392)
Key features and details
- Rabbit polyclonal to Plasma Kallikrein 1B
- Suitable for: ELISA, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Plasma Kallikrein 1B antibody
See all Plasma Kallikrein 1B primary antibodies -
Description
Rabbit polyclonal to Plasma Kallikrein 1B -
Host species
Rabbit -
Specificity
This antibody is specific for Plasma Kallikrein 1B (amino end of the catalytic domain). -
Tested applications
Suitable for: ELISA, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
A synthetic peptide based on the amino end of the catalytic domain of human Plasma Kallikrein 1B.
Read Abcam's proprietary immunogen policy (Peptide available as ab43118.) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab44392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | (1) |
Use at an assay dependent concentration.
|
| IHC-P | (1) |
Use at an assay dependent concentration.
|
| WB | (4) |
Use at an assay dependent concentration. Predicted molecular weight: 71 kDa.
1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. |
| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 71 kDa. 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. |
Target
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Function
The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. -
Involvement in disease
Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect. -
Sequence similarities
Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
Contains 4 apple domains.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3818 Human
- Entrez Gene: 16621 Mouse
- Omim: 229000 Human
- SwissProt: P03952 Human
- SwissProt: P26262 Mouse
- Unigene: 237642 Human
- Unigene: 482691 Mouse
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Alternative names
- Fletcher factor antibody
- kallikrein B plasma antibody
- Kallikrein B plasma (Fletcher factor) 1 antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab44392 has been referenced in 1 publication.
- Pihl R et al. ITIH4 acts as a protease inhibitor by a novel inhibitory mechanism. Sci Adv 7:N/A (2021). PubMed: 33523981
