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  1. Link

    plasma-kallikrein-1b-antibody-ab44392.pdf

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Cell Biology Proteolysis / Ubiquitin Proteolytic enzymes Serine protease Kallikreins
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Anti-Plasma Kallikrein 1B antibody (ab44392)

  • Datasheet
Reviews (6)Q&A (4)

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  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody (ab44392)

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Primary

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Secondary

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Protein

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-Plasma Kallikrein 1B antibody
    See all Plasma Kallikrein 1B primary antibodies
  • Description

    Rabbit polyclonal to Plasma Kallikrein 1B
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for Plasma Kallikrein 1B (amino end of the catalytic domain).
  • Tested applications

    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide based on the amino end of the catalytic domain of human Plasma Kallikrein 1B.

    Read Abcam's proprietary immunogen policy (Peptide available as ab43118.)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Serine protease
    • Kallikreins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Rat Plasma Kallikrein 1B protein (His tag) (ab226435)

Applications

Our Abpromise guarantee covers the use of ab44392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 71 kDa. 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.

Target

  • Function

    The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
  • Involvement in disease

    Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect.
  • Sequence similarities

    Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
    Contains 4 apple domains.
    Contains 1 peptidase S1 domain.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P03952 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3818 Human
    • Entrez Gene: 16621 Mouse
    • Omim: 229000 Human
    • SwissProt: P03952 Human
    • SwissProt: P26262 Mouse
    • Unigene: 237642 Human
    • Unigene: 482691 Mouse
    • Alternative names

      • Fletcher factor antibody
      • kallikrein B plasma antibody
      • Kallikrein B plasma (Fletcher factor) 1 antibody
      • Kallikrein B, plasma 1 antibody
      • Kallikrein B1 antibody
      • Kininogenin antibody
      • KLK3 antibody
      • KLKB1 antibody
      • KLKB1_HUMAN antibody
      • PKK antibody
      • PKKD antibody
      • Plasma kallikrein antibody
      • Plasma kallikrein B1 antibody
      • Plasma kallikrein light chain antibody
      • Plasma prekallikrein antibody
      • PPK antibody
      • Prekallikrein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody (ab44392)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody (ab44392)This image is courtesy of an anonymous Abreview
      ab44392 staining Kallikrein 1B in human temporal neocortical tissue sections IHC-P . Tissue samples were fixed with formaldehyde and blocked with 10% serum for 20 minutes at 23°C: antigen retrieval was performed by heat mediation in citrate buffer.. Samples were incubated with primary antibody (1/100) for 18 hours at 23°C . A biotin-conjugated horse anti Rabbit IgG  polyclonal (1/50) was used as secondary antibody.

      See Abreview

    Protocols

    • Recommended WB protocol

    Datasheets and documents

    • Datasheet
  • References

    ab44392 has not yet been referenced specifically in any publications.

    Publishing research using ab44392? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-6 of 6 Abreviews

    Western blot abreview for Anti-Plasma Kallikrein 1B antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Purified protein (Plasma)
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE gel)
    Loading amount
    2 µg
    Specification
    Plasma
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    匡 勅使川原

    Verified customer

    Submitted Jun 19 2018

    Western blot abreview for Anti-Plasma Kallikrein 1B antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Pig Purified protein (porcine plasma, porcine articular capsule extract)
    Gel Running Conditions
    Non-reduced Denaturing (10% PAGE)
    Loading amount
    0.2 µg
    Specification
    porcine plasma, porcine articular capsule extract
    Blocking step
    Tween 20 as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 10 2016

    ELISA abreview for Anti-Plasma Kallikrein 1B antibody

    Good
    Abreviews
    Abreviews
    Application
    ELISA
    Sample
    Human Cell (human brain)
    Specification
    human brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 0.5% · Temperature: 22°C
    Type
    Sandwich (Detection)
    Read More

    Miss. Emma Ashby

    Verified customer

    Submitted Dec 10 2010

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Plasma Kallikrein 1B antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (temporal neocortex)
    Specification
    temporal neocortex
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 26 2009

    Western blot abreview for Anti-Plasma Kallikrein 1B antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Purified protein (human plasma)
    Loading amount
    4 µg
    Specification
    human plasma
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 26 2009

    Western blot abreview for Anti-Plasma Kallikrein 1B antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (blood)
    Loading amount
    20 µg
    Specification
    blood
    Gel Running Conditions
    Reduced Denaturing (4-20% gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 16 2009

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