Anti-Plasma Kallikrein 1B antibody (ab44392)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Plasma Kallikrein 1B antibody
See all Plasma Kallikrein 1B primary antibodies -
Description
Rabbit polyclonal to Plasma Kallikrein 1B -
Host species
Rabbit -
Specificity
This antibody is specific for Plasma Kallikrein 1B (amino end of the catalytic domain). -
Tested applications
Suitable for: ELISA, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
A synthetic peptide based on the amino end of the catalytic domain of human Plasma Kallikrein 1B.
Read Abcam's proprietary immunogen policy (Peptide available as ab43118.)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab44392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | 1/1000. Predicted molecular weight: 71 kDa. 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. |
Target
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Function
The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. -
Involvement in disease
Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect. -
Sequence similarities
Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
Contains 4 apple domains.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3818 Human
- Entrez Gene: 16621 Mouse
- Omim: 229000 Human
- SwissProt: P03952 Human
- SwissProt: P26262 Mouse
- Unigene: 237642 Human
- Unigene: 482691 Mouse
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Alternative names
- Fletcher factor antibody
- kallikrein B plasma antibody
- Kallikrein B plasma (Fletcher factor) 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody (ab44392)This image is courtesy of an anonymous Abreviewab44392 staining Kallikrein 1B in human temporal neocortical tissue sections IHC-P . Tissue samples were fixed with formaldehyde and blocked with 10% serum for 20 minutes at 23°C: antigen retrieval was performed by heat mediation in citrate buffer.. Samples were incubated with primary antibody (1/100) for 18 hours at 23°C . A biotin-conjugated horse anti Rabbit IgG polyclonal (1/50) was used as secondary antibody.
Protocols
Datasheets and documents
References
ab44392 has not yet been referenced specifically in any publications.
