Western blot abreview for Anti-Plasma Kallikrein 1B antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (blood)
Loading amount
20 µg
Specification
blood
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/2000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 75 kDa Non-specific: 60, 50, 25 kDa

Additional data

Additional Notes
Loading Amount is 1 ul whole blood.

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Submitted Jun 16 2009

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