Western blot abreview for Anti-Plasma Kallikrein 1B antibody

Good
Abreviews
Application
Western blot
Sample
Human Purified protein (human plasma)
Loading amount
4 µg
Specification
human plasma
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 5% Milk

Secondary antibody

Name
Non-Abcam antibody was used: Vector Peroxidase anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
milipore ECL
Exposure
40 second(s)
Bands
Specific: 20-35 kDa

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Submitted Oct 26 2009

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