Western blot abreview for Anti-Plasma Kallikrein 1B antibody

Excellent
Abreviews
Application
Western blot
Sample
Rat Purified protein (Plasma)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE gel)
Loading amount
2 µg
Specification
Plasma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA, PBS
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Anti-IgG (H+L chain) (Rabbit) pAb-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
3 second(s)
Bands
Specific: 24 kDa
Positive control
Prekallikrein, Human Plasma (Merck #529583) Natural Human Plasma Kallikrein 1B Protain (Abcam #ab77870) Natural Human Plasma Kallikrein 1B Protain (Abcam #ab187005)

匡 勅使川原

Verified customer

Submitted Jun 19 2018

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