Product nameAnti-Plasma Kallikrein 1B antibody [EPR5707]
See all Plasma Kallikrein 1B primary antibodies
DescriptionRabbit monoclonal [EPR5707] to Plasma Kallikrein 1B
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human Plasma Kallikrein 1B aa 600-700 (C terminal). The exact sequence is proprietary.
- Human breast cancer, Human plasma, Human ovary and fetal liver lysates; Human kidney tissue
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.09 x 10 -10 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 71 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionThe enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
Involvement in diseaseDefects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect.
Sequence similaritiesBelongs to the peptidase S1 family. Plasma kallikrein subfamily.
Contains 4 apple domains.
Contains 1 peptidase S1 domain.
- Information by UniProt
- Fletcher factor antibody
- kallikrein B plasma antibody
- Kallikrein B plasma (Fletcher factor) 1 antibody
All lanes : Anti-Plasma Kallikrein 1B antibody [EPR5707] (ab124938) at 1/1000 dilution
Lane 1 : Human breast cancer lysate
Lane 2 : Human plasma lysate
Lane 3 : Human ovary lysate
Lane 4 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 71 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
ab124938, at 1/50 dilution, staining Plasma Kallikrein 1B in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124938 has not yet been referenced specifically in any publications.