Recombinant
RabMAb

Anti-Plasma Kallikrein 1B antibody [EPR5707] (ab124938)

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Overview

  • Product name
    Anti-Plasma Kallikrein 1B antibody [EPR5707]
    See all Plasma Kallikrein 1B primary antibodies
  • Description
    Rabbit monoclonal [EPR5707] to Plasma Kallikrein 1B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Plasma Kallikrein 1B aa 600-700 (C terminal). The exact sequence is proprietary.

  • Positive control
    • Human breast cancer, Human plasma, Human ovary and fetal liver lysates; Human kidney tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 71 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
  • Involvement in disease
    Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect.
  • Sequence similarities
    Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
    Contains 4 apple domains.
    Contains 1 peptidase S1 domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • Fletcher factor antibody
      • kallikrein B plasma antibody
      • Kallikrein B plasma (Fletcher factor) 1 antibody
      • Kallikrein B, plasma 1 antibody
      • Kallikrein B1 antibody
      • Kininogenin antibody
      • KLK3 antibody
      • KLKB1 antibody
      • KLKB1_HUMAN antibody
      • PKK antibody
      • PKKD antibody
      • Plasma kallikrein antibody
      • Plasma kallikrein B1 antibody
      • Plasma kallikrein light chain antibody
      • Plasma prekallikrein antibody
      • PPK antibody
      • Prekallikrein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [EPR5707] (ab124938)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [EPR5707] (ab124938)
      ab124938, at 1/50 dilution, staining Plasma Kallikrein 1B in paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • Western blot - Anti-Plasma Kallikrein 1B antibody [EPR5707] (ab124938)
      Western blot - Anti-Plasma Kallikrein 1B antibody [EPR5707] (ab124938)
      All lanes : Anti-Plasma Kallikrein 1B antibody [EPR5707] (ab124938) at 1/1000 dilution

      Lane 1 : Human breast cancer lysate
      Lane 2 : Human plasma lysate
      Lane 3 : Human ovary lysate
      Lane 4 : Fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 71 kDa
      Observed band size: 80 kDa (why is the actual band size different from the predicted?)

    • Other - Anti-Plasma Kallikrein 1B antibody [EPR5707] (ab124938)
      Other - Anti-Plasma Kallikrein 1B antibody [EPR5707] (ab124938)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab124938 has not yet been referenced specifically in any publications.

    Publishing research using ab124938? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Review or Q&A

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    Votes: Highest First

    Hello,

    Can you tell me what is the actual quantity (e.g. in micrograms) of antibody that is included in the rabbit monoclonal anti-plasmakallikrein1B (ab124938)?

    The size says only 100 ul, and while it gives recommended dilutions ...

    Read More

    Thank you for contacting us with your question.

    Ab124938 is provided as unpurified tissue culture supernatant, and since there other proteins present in this solution, it isn't possible to measure the concentration of the specific antibody. S...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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