Recombinant
RabMAb

Recombinant Anti-Plasma Kallikrein 1B antibody [EPR5707] (ab124938)

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Overview

  • Product name

    Anti-Plasma Kallikrein 1B antibody [EPR5707]
    See all Plasma Kallikrein 1B primary antibodies

  • Description

    Rabbit monoclonal [EPR5707] to Plasma Kallikrein 1B

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human Plasma Kallikrein 1B aa 600-700 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human breast cancer, Human plasma, Human ovary and fetal liver lysates; Human kidney tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 1.09 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR5707

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 71 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.

  • Involvement in disease

    Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect.

  • Sequence similarities

    Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
    Contains 4 apple domains.
    Contains 1 peptidase S1 domain.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • Fletcher factor antibody
      • kallikrein B plasma antibody
      • Kallikrein B plasma (Fletcher factor) 1 antibody
      • Kallikrein B, plasma 1 antibody
      • Kallikrein B1 antibody
      • Kininogenin antibody
      • KLK3 antibody
      • KLKB1 antibody
      • KLKB1_HUMAN antibody
      • PKK antibody
      • PKKD antibody
      • Plasma kallikrein antibody
      • Plasma kallikrein B1 antibody
      • Plasma kallikrein light chain antibody
      • Plasma prekallikrein antibody
      • PPK antibody
      • Prekallikrein antibody
      see all

    Images

    • Western blot - Anti-Plasma Kallikrein 1B antibody [EPR5707] (ab124938)
      Western blot - Anti-Plasma Kallikrein 1B antibody [EPR5707] (ab124938)
      All lanes : Anti-Plasma Kallikrein 1B antibody [EPR5707] (ab124938) at 1/1000 dilution

      Lane 1 : Human breast cancer lysate
      Lane 2 : Human plasma lysate
      Lane 3 : Human ovary lysate
      Lane 4 : Fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 71 kDa
      Observed band size: 80 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [EPR5707] (ab124938)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [EPR5707] (ab124938)

      ab124938, at 1/50 dilution, staining Plasma Kallikrein 1B in paraffin-embedded Human kidney tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Other - Anti-Plasma Kallikrein 1B antibody [EPR5707] (ab124938)
      Other - Anti-Plasma Kallikrein 1B antibody [EPR5707] (ab124938)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab124938 has not yet been referenced specifically in any publications.

    Publishing research using ab124938? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    Hello,

    Can you tell me what is the actual quantity (e.g. in micrograms) of antibody that is included in the rabbit monoclonal anti-plasmakallikrein1B (ab124938)?

    The size says only 100 ul, and while it gives recommended dilutions for some purposes, I want to use it for another purpose-- i.e. to covalently conjugate it to magnetic beads for a bead-based IP, and for this process, I need to know the absolute quantity of antibody. Thanks,

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    Answer

    Thank you for contacting us with your question.

    Ab124938 is provided as unpurified tissue culture supernatant, and since there other proteins present in this solution, it isn't possible to measure the concentration of the specific antibody. So unfortunately we do not know the mass of antibody in the 100 uL provided. Each new lot is tested and adjusted so that the optimal results are seen using the dilutions listed on the datasheet.

    It is possibleto purify the IgG molecules out of the supernatant using a protein A or G purification and then measuring the concentration, however the yield will be very low with only 100 uL (we recommend 10-50 mL for our supernatant purification kits like ab109207). So, if the mass or concentration is essential information for your assay, I would recommend using a purified antibody.

    We have several other plasma kallikrein 1B antibodies such as ab1006 and ab44392, and though these haven't been validated in IP they may work in this application. We do have a testing discount program if you'd be interested in trying on of these other antibodies in your application.

    I am sorry that we don't have the information that you're looking for, but if you have any further questions or if there is anything else that we can do for you, please let me know and I'll be happy to help.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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