Key features and details
- Sample type: Cell culture media, Tissue Culture Media
Product namePlasma Membrane Isolation Kit
See all Cell Plasma Membrane kits
Sample typeCell culture media, Tissue Culture Media
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mammals
This Plasma Membrane Isolation Kit (ab284937) (previously known as OrgFrontier Plasma Membrane Isolation Kit K414) provides a proprietary set of reagents and buffers designed to enable optimized extraction and isolation of intact plasma membrane from mammalian tissues and cells.
The protocol requires an ultracentrifuge to be performed and can be completed in a few hours.
This product is manufactured by BioVision, an Abcam company and was previously called K414 OrgFrontier™ Plasma Membrane Isolation Kit. K414-10 is the same size as the 10 test size of ab284937.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 10 tests EZBlock Protease Inhibitor Cocktail Orange cap 1 x 1ml Gradient Dilution Buffer NM 1 x 100ml Homogenization Buffer NM 1 x 50ml OptiPrep Density Gradient Medium WM 1 x 85ml
Cholesterol content by fraction, assayed using Cholesterol/ Cholesteryl Ester Assay Kit - Quantitation (ab65359). Cholesterol is highly enriched in the plasma membrane (approximately 22% of the total lipid content of the plasma membrane), but is found in much lower concentration (2%-6% of total lipids) in other organelle membranes.
ab284937 has not yet been referenced specifically in any publications.