• Product name
    Plasma Membrane Protein Extraction Kit
  • Assay type
  • Product overview

    The Plasma Membrane Protein Extraction Kit provides optimized buffers and reagents for effective extraction of membrane proteins from mammalian tissues and cells. Unlike other available procedures that can only extract the total cellular membrane proteins (combinations of plasma and organelle membrane proteins), this kit was designed to not only extract the total cellular membrane proteins, but also purify the plasma membrane proteins specifically. The procedure offers consistent yield and high purity (over 90%). Membrane proteins prepared using the kit can be utilized in a variety of applications, such as Western blotting, 2-D gels, and enzyme analyses, etc. The entire procedure takes less than 1 hour.

  • Tested applications
    Suitable for: Purificationmore details


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 50 tests
    Homogenize Buffer NM 1 x 100ml
    Lower Phase Solution WM 1 x 20ml
    Protease Inhibitor Cocktail (Lyophilised) Red 1 vial
    Upper Phase Solution NM 1 x 20ml
  • Research areas
  • Relevance
    Membrane Protein Extraction is a method for extraction of membrane proteins from mammalian tissues and cells. Many procedures extract total cellular membrane proteins which is a combination of plasma and organelle membrane proteins.


Our Abpromise guarantee covers the use of ab65400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Purification Use at an assay dependent concentration.


  • Cell membrane protein was collected using plasma membrane protein extraction kit (ab65400). The upper panels show the plasma membrane myc-tagged protein; the middle panels show the cytosolic myc-tagged proteins and the lower panels show the total myc-tagged protein from CHO (Chinese Hamster ovary) whole-cell lysates.



This product has been referenced in:
  • Wang H  et al. Targeted production of reactive oxygen species in mitochondria to overcome cancer drug resistance. Nat Commun 9:562 (2018). Read more (PubMed: 29422620) »
  • Das S  et al. Intranasally delivered small interfering RNA-mediated suppression of scavenger receptor Mac-1 attenuates microglial phenotype switching and working memory impairment following hypoxia. Neuropharmacology 137:240-255 (2018). Read more (PubMed: 29738851) »

See all 37 Publications for this product

Customer reviews and Q&As

Thank you for the update. I am glad to hear the replacement kit worked out better for you. Eliminating those extra steps will probably, as you found, give purer fractions but possibly at the cost of lower yield.

I am not sure why tubulin was g...

Read More

Merci pour votre requête. J'ai contacté le laboratoire et ils m'ont confirmé que cette étape est pour équilibrer la "upper phase" solution. Il ne faux donc pas prendre la solution directement de la bouteille....

Read More

This kit may be useful for extracting and retainingthe plasma membrane fraction without the plasma membrane proteins but it has not been specifically designed for this purpose. Please let us know how things go if you do choose to use ab65400 for this e...

Read More

I can confirm that the kit uses an aqueous polymer two-phase system to separate plasma membranes. Most specifically it uses the PEG/ Dextran system and the plasma membrane tend to concentrate in the upper phase.

I am sorry we do not have ...

Read More

1. The smaller cell number can decrease yield. Hence it is at the user's discretion to reduce cell numbers. For some cells, based on size and density of expression of membrane proteins, smaller cell number can still yield enough membrane proteins. If c...

Read More

1. This kit isolates the plasma membrane associated proteins as well as transmembrane proteins on the plasma membrane. Organelle membrane proteins are not isolated. If total membrane proteins were desired, you would get all membrane proteins ...

Read More

Yes, this will give you two sub-cellular fractions: plasma membrane and cytosolic. So you will be able to demonstrate the relative amounts of your protein of interest in each fraction using an assay such as western blotting, assuming you have an antibo...

Read More

The proteins isolated when using this kit should be in their native conformation.

The frozen tissue cannot be pulverized with the Dounce homogenizer. This has to be done with a pulverizer or tissue grinder. Once ground, the buffer can be used to homogenize the bits in a Dounce homogenizer.

Fresh tissue can be homogenized di...

Read More

You are obtaining a huge pellet after step 4. This is the low speed centrifugation at 700g and pellets down unbroken cells, nuclei and debris. Getting a huge pellet at this step is indicative of incomplete homogenization. The steps are correct but...

Read More

1-10 of 88 Abreviews or Q&A


Sign up