Overview

  • Product name

    Plasmin Activity Assay Kit (Fluorometric)
  • Detection method

    Fluorescent
  • Sample type

    Plasma, Purified protein
  • Assay type

    Enzyme activity
  • Sensitivity

    10 ng
  • Assay time

    0h 20m
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Plasmin Activity Assay Kit (Fluorometric) (ab204728) is based on the ability of Plasmin to proteolytically cleave a synthetic plasmin substrate and release a fluorophore, AMC, which can be easily quantified by fluorescence microplate readers at Ex/Em = 360/450 nm. This assay kit is simple, rapid and can detect plasmin activity as low as 10 ng in a variety of samples.

  • Notes

    Plasmin (EC 3.4.21.7) is a serine protease occurring in plasma as plasminogen. Upon activation via cleavage by plasminogen activators; plasmin solubilizes fibrin clots and activates and/or degrades compounds of the coagulation and complement systems. Plasminogen-Plasmin system has also been implicated in a wide variety of physiologic and pathologic processes, including tumor growth, invasion and metastasis.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Plasmin Assay Buffer 1 x 15ml
    Plasmin Dilution Buffer 1 x 1.5ml
    Plasmin Enzyme Standard (1 mg/ml) 1 x 5µl
    Plasmin Substrate 1 x 200µl
  • Research areas

  • Function

    Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
    Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.
  • Tissue specificity

    Present in plasma and many other extracellular fluids. It is synthesized in the liver.
  • Involvement in disease

    Plasminogen deficiency
  • Sequence similarities

    Belongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 5 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.
  • Domain

    Kringle domains mediate interaction with CSPG4.
  • Post-translational
    modifications

    N-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
    In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide.
  • Cellular localization

    Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface.
  • Information by UniProt
  • Alternative names

    • Plasmin light chain B
    • Plasminogen
    • PLG
    • PLMN_HUMAN

Images

  • Typical Plasmin standard curve.

  • Plasmin activity was measured in plasma samples in the presence and absence of a Plasmin inhibitor, Aprotinin. S = Substrate, I = Inhibitor, AB = Activation Buffer containing Urokinase

Protocols

References

ab204728 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Plasmin activity in human urine

Good Excellent 5/5 (Ease of Use)
Abreviews
The assay kit is actually quite straightforward to use. The recommended standard curve worked, though we included a few lower values to determine the detection limit of the assay (6.25 ng to 250 ng). The kit was able to detect plasmin activity in human urine, though the activity levels were relatively low (but within the standard curve).

Abcam user community

Verified customer

Submitted Oct 03 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up