Overview

  • Product name
    Anti-Plasmin antibody (Biotin)
  • Description
    Rabbit polyclonal to Plasmin (Biotin)
  • Host species
    Rabbit
  • Conjugation
    Biotin
  • Specificity
    This antibody does recognize plasminogen at about 20-40 %.
  • Tested applications
    Suitable for: RIA, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) - plasmin purified from human plasma.

Properties

Applications

Our Abpromise guarantee covers the use of ab48350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
RIA Use at an assay dependent dilution.
WB Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    Plasmin is an enzyme formed in the circulating blood, and many other extracellular fluids, from the zymogen plasminogen. Plasminogen is a single-chain glycoprotein with 790 amino acid residues. Activation to the active form, plasmin, by urokinase involves cleavage at the arg-val bond between residues 560 and 561, resulting in the formation of the 2-chain plasmin molecule held together by 2 disulfide linkages. The heavier chain contains about 411 residues and the lighter chain about 233. The main function of plasmin is the digestion of fibrin in blood clots. Plasmin is a proteolytic enzyme with a specificity similar to that of trypsin. Like trypsin, plasmin belongs to the family of serine proteinases, in which the active site catalytic triad, his-57, asp-102, and ser-195 (chymotrypsin numbering), is situated in the light chain. Plasmin acts as a proteolytic factor in a variety of processes other than fibrinolysis; including embryonic development, tissue remodelling, tumour invasion and inflammation; in ovulation it weakens the walls of the Graafian follicle. A Deficiency of plasmin may lead to thrombosis, as clots are not degraded adequately.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • Plasminogen antibody
    • PLG antibody

References

This product has been referenced in:
  • Leksa V  et al. Dissecting mannose 6-phosphate/insulin-like growth factor 2 receptor complexes that control activation and uptake of plasminogen in cells. J Biol Chem : (2012). WB . Read more (PubMed: 22613725) »
  • Barker R  et al. Plasminogen and plasmin in Alzheimer's disease. Brain Res 1355:7-15 (2010). WB ; Human . Read more (PubMed: 20709033) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for contacting us.

We currently offer only one anti-Plasmin antibody ab48350. This product has not been tested as a neutralizing/blocking antibody for plasmin. As a rabbit polyclonal this product will recognize a number of epitopes on plasmin and therefore may neutralize it, however there will also be lot to lot variation in epitope recognition.



For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Read More

Answer

Merci pour votre patience. Je m'excuse pour ce délais.

Je peux confirmer que cet anticorps était produit contre la plasmine et reconnait aussi le plasminogène à environ 20 à 40% d'intensité.

Je peux aussi confirmer que la reconnaissance des chaînes lourdes et légères n'a pas été testé séparément.

Pour ce qui est de la reconnaissance de la plasmine complexé, le laboratoire n'as pas testé ceci et peux que faire des prédictions. Dans les deux cas c'est possible que cet anticorps reconnait la forme complexé, soit à la PAI soit la PNA complexé à travers des groupes carboxy.

J'espère que ces informations vous sont toujours utiles. N'hésitez pas à nous recontacter si vous avez des autres questions.

Veuillez aussi nous recontacter si vous êtes toujours intéressez de tester cet anticorps. Je pourrais éventuellement vous faire une offre de test.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Purified protein (Blood)
Loading amount
3 µg
Specification
Blood
Gel Running Conditions
Reduced Denaturing (4-20% Tris HCl gels)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Rachel Barker

Verified customer

Submitted Aug 10 2009

Answer

Thank you for your enquiry. This antibody has not been tested if they can recognize plasminogen. Therefore, I can not confirm if it only detects plasmin. This antibody reacts 15% to mouse sample, which means that the remaining 85% is no reaction.

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Question
Answer

Thank you for your enquiry. This antibody has been tested to full length human plasmin. It cross reacts with rat plasmin 10%, and mouse plasmin 15%.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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