Plasmin Inhibitor Screening Assay Kit (Fluorometric) (ab204729)

Overview

  • Product name

    Plasmin Inhibitor Screening Assay Kit (Fluorometric)
  • Detection method

    Fluorescent
  • Sample type

    Inhibitor compounds
  • Assay type

    Enzyme activity
  • Product overview

    Plasmin Inhibitor Screening Assay Kit (Fluorometric) (ab204729) is based on the ability of Plasmin to cleave a synthetic AMC-based peptide substrate to release AMC, which can be detected by measuring its fluorescence at Ex/Em = 360/450 nm. In the presence of Plasmin specific inhibitors, the extent of cleavage reaction is reduced or completely abolished. The loss in the fluorescence intensity can be correlated to the amount of inhibitor present in the assay solution. The kit provides a simple and rapid method to screen potential inhibitors of Plasmin.

  • Notes

    Plasmin (EC 3.4.21.7) is a serine protease occurring in plasma as plasminogen. Upon activation via cleavage by plasminogen activators; plasmin solubilizes fibrin clots and activates and/or degrades compounds of the coagulation and complement systems. Plasmin inhibitors are critical in the treatment of hyperfibrinolysis-associated blood loss and related complications.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Plasmin Assay Buffer 1 x 15ml
    Plasmin Dilution Buffer 1 x 1.5ml
    Plasmin Enzyme 1 x 15µl
    Plasmin Inhibitor (Aprotinin, 0.6 mM) 1 x 100µl
    Plasmin Substrate 1 x 200µl

Images

  • Inhibition of Plasmin activity by a Plasmin Inhibitor (Aprotinin).

  • Plasmin activity was measured in plasma samples in the presence and absence of a Plasmin inhibitor, Aprotinin. S = Substrate, I = Inhibitor, AB = Activation Buffer containing Urokinase.

Protocols

References

ab204729 has not yet been referenced specifically in any publications.

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