Plasminogen activator Inhibitor Type 1 Human Chromogenic Activity Assay Kit (ab108894)

Overview

  • Product name

    Plasminogen activator Inhibitor Type 1 Human Chromogenic Activity Assay Kit
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Plasma
  • Assay type

    Quantitative
  • Sensitivity

    0.5 IU/ml
  • Range

    0.625 IU/ml - 20 IU/ml
  • Product overview

    Plasminogen activator Inhibitor Type 1 Human Chromogenic Activity Kit (ab108894) has been developed to determine human type I plasminogen activator inhibitor (PAI-1) activity in cell culture supernatants. A fixed amount of tissue-type plasminogen activator (tPA) is added in excess to undiluted sample, which allows PAI-1 and tPA to form an inactive complex. The assay measures plasminogen activation by residual tPA in coupled assays that contain tPA, plasminogen, and a plsmin-specific synthetic substrate. The amount of plasmin produced is quantitated using a highly specific plasmin substrate releasing a yellow para-nitroaniline (pNA) chromophore. The absorbance of the pNA at 405 nm is inversely proportional to the PAI-1 enzymatic activity.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Notes

    Type I plasminogen activator inhibitor (PAI-1) is a 50 kDa serpin family member that inhibits tissue- and urokinase-type plaminogen activators (t-PA, u-PA). This protein appears to be an important regulator of plasminogen activation by t-PA and extracellular proteolysis by u-PA. The plasminogen activator proteolytic enzyme systems are important not only for fibrinolysis but also for extracellular matrix remodeling, and have been implicated in a number of normal and pathological processes including angiogensis, ovulation and embryogenesis, thrombotic and hemorrhagic disorders, connective tissue diseases, neoplasm and sepsis. PAI-1 is a prognosticator in breast cancer, gastric cancer, various forms of lung cancer and cervical cancer.

  • Platform

    Microplate reader

Properties

Images

Protocols

References

ab108894 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for getting in touch.


I can confirm that the tPA standard must be added to all the sample including cell culture supernatant for the Plasminogen activator Inhibitor Type 1 Human Chromogenic Activity Kit (ab108894).


I am very sorry about this mistake in our protocol booklet. Thank you that you brought it to our attention and our team is going is updating it at the moment.


Below is the information from the lab:


"The tPA standard need to be added for all the sample since the PAI activity will be evaluated by inhibit the tPA activity.  You could add your cell culture sample without dilution to see how much they can inhibit the tPA activity.  Ideally, you will do a series dilution of your cell culture sample to see how the dilution will affect the tPA activity and so that you can see the full picture of the inhibition."


Since the amount of tPA standard to be added to cell culture supernatant is not strictly determined, you might need to do some pilot experiments to find the best condition. 


Please do not hesitate to let me know if you have any other questions.

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Question
Answer

Our lab has found that rat and mouse samples both show no significant activity with this particular kit. We would not advise using mouse or rat samples.

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Answer

Muchas gracias por contactarnos.
Todas las referencias, publicaciones, e información disponible sobre nuestros productos la compartimos en nuestra web para utilidad de los usuarios.
Lamentablemente para este kit no tenemos ninguna referencia disponible, ni ninguna publicación (Abreview) de otros usuarios.
Sin embargo, todos los productos de Abcam tienen 6 meses de garantía (Abpromise), y en caso de no funcionar tal y como se especifica en la datasheet, se os reemplaza el producto por el mismo u otro diferente, o bien se os devuelve el importe del mismo.
También agradecemos cualquier feedback acerca de nuestros productos que nos podáis dar. Si quieres ser el primero en mandar un Abreview acerca de este kit, puedes beneficiarte de puntos canjeables por promociones y descuentos. Encontrarás más información en :
https://www.abcam.com/index.html?pageconfig=resource&rid=10332&viapagetrap=abreviews
Siento no poder ser de más ayuda. Si tienes cualquier otra consulte no dudes en contactarnos de nuevo, y estaremos encantados de poder ayudar.

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