Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell culture supernatant, Plasma
- Sensitivity: 0.5 IU/ml
Product namePlasminogen activator Inhibitor Type 1 Human Chromogenic Activity Assay Kit
Sample typeCell culture supernatant, Plasma
Range0.625 IU/ml - 20 IU/ml
Plasminogen activator Inhibitor Type 1 Human Chromogenic Activity Kit (ab108894) has been developed to determine human type I plasminogen activator inhibitor (PAI-1) activity in cell culture supernatants. A fixed amount of tissue-type plasminogen activator (tPA) is added in excess to undiluted sample, which allows PAI-1 and tPA to form an inactive complex. The assay measures plasminogen activation by residual tPA in coupled assays that contain tPA, plasminogen, and a plasmin-specific synthetic substrate. The amount of plasmin produced is quantitated using a highly specific plasmin substrate releasing a yellow para-nitroaniline (pNA) chromophore. The absorbance of the pNA at 405 nm is inversely proportional to the PAI-1 enzymatic activity.
Upon arrival, immediately store components of the kit at recommended temperatures. Avoid repeated freeze-thaw cycles.
Type I plasminogen activator inhibitor (PAI-1) is a 50 kDa serpin family member that inhibits tissue- and urokinase-type plaminogen activators (t-PA, u-PA). This protein appears to be an important regulator of plasminogen activation by t-PA and extracellular proteolysis by u-PA. The plasminogen activator proteolytic enzyme systems are important not only for fibrinolysis but also for extracellular matrix remodeling, and have been implicated in a number of normal and pathological processes including angiogensis, ovulation and embryogenesis, thrombotic and hemorrhagic disorders, connective tissue diseases, neoplasm and sepsis. PAI-1 is a prognosticator in breast cancer, gastric cancer, various forms of lung cancer and cervical cancer.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Assay Diluent 1 x 30ml Human Plasminogen (Lyophilized) 1 vial Microplate 1 unit Plasmin Substrate (Lyophilized) 1 vial Sealing Tapes 3 units tPA Standard 100IU (Lyophilized) 1 vial
- Clade E
- Endothelial plasminogen activator inhibitor
ab108894 has not yet been referenced specifically in any publications.