RecombinantRabMAb

Recombinant Anti-Plasminogen antibody [EPR22406-159] (ab245247)

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Western blot - Anti-Plasminogen antibody [EPR22406-159] (ab245247)
  • Immunoprecipitation - Anti-Plasminogen antibody [EPR22406-159] (ab245247)
  • Anti-Plasminogen antibody [EPR22406-159] (ab245247)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22406-159] to Plasminogen
  • Suitable for: WB, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Plasminogen antibody [EPR22406-159]
    See all Plasminogen primary antibodies

  • Description

    Rabbit monoclonal [EPR22406-159] to Plasminogen

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: IHC-Fr or IHC-P

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment within Mouse Plasminogen aa 1-100. The exact sequence is proprietary.
    Database link: P20918

  • Positive control

    • WB: Mouse serum and plasma; Mouse liver lysate. IP: Mouse liver lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab245247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 91 kDa).
IP 1/30.
  • Application notes
    Is unsuitable for IHC-Fr or IHC-P.

    • Target

    • Function

      Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
      Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.

    • Tissue specificity

      Present in plasma and many other extracellular fluids. It is synthesized in the liver.

    • Involvement in disease

      Defects in PLG are a cause of susceptibility to thrombosis (THR) [MIM:188050]. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation.
      Defects in PLG are the cause of plasminogen deficiency (PLGD) [MIM:217090]. PLGD is characterized by decreased serum plasminogen activity. Two forms of the disorder are distinguished: type 1 deficiency is additionally characterized by decreased plasminogen antigen levels and clinical symptoms, whereas type 2 deficiency, also known as dysplasminogenemia, is characterized by normal, or slightly reduced antigen levels, and absence of clinical manifestations. Plasminogen deficiency type 1 results in markedly impaired extracellular fibrinolysis and chronic mucosal pseudomembranous lesions due to subepithelial fibrin deposition and inflammation. The most common clinical manifestation of type 1 deficiency is ligneous conjunctivitis in which pseudomembranes formation on the palpebral surfaces of the eye progresses to white, yellow-white, or red thick masses with a wood-like consistency that replace the normal mucosa.

    • Sequence similarities

      Belongs to the peptidase S1 family. Plasminogen subfamily.
      Contains 5 kringle domains.
      Contains 1 PAN domain.
      Contains 1 peptidase S1 domain.

    • Domain

      Kringle domains mediate interaction with CSPG4.

    • Post-translational
      modifications

      N-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
      In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide.

    • Cellular localization

      Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface.
    • Information by UniProt

    • Database links

      • Form

        Cleaved into the following 5 chains: 1.Plasmin heavy chain A2.Activation peptide3.Angiostatin4.Plasmin heavy chain A, short form5. Plasmin light chain B

      • Alternative names

        • Plasmin antibody
        • Plasmin heavy chain A antibody
        • Plasmin light chain B antibody
        • Plasminogen antibody
        • PLG antibody
        • PLMN_HUMAN antibody
        see all

      Images

      • Western blot - Anti-Plasminogen antibody [EPR22406-159] (ab245247)
        Western blot - Anti-Plasminogen antibody [EPR22406-159] (ab245247)
        All lanes : Anti-Plasminogen antibody [EPR22406-159] (ab245247) at 1/1000 dilution

        Lane 1 : Mouse serum
        Lane 2 : Mouse plasma
        Lane 3 : Mouse liver lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

        Predicted band size: 91 kDa
        Observed band size: 97 kDa
        why is the actual band size different from the predicted?



        Exposure time : Lane 1:15 seconds; Lane 2:5 seconds; Lane 3:3 minutes.

        Blocking/Dilution buffer: 5% NFDM/TBST.

      • Immunoprecipitation - Anti-Plasminogen antibody [EPR22406-159] (ab245247)
        Immunoprecipitation - Anti-Plasminogen antibody [EPR22406-159] (ab245247)

        Plasminogen was immunoprecipitated from 0.35 mg of mouse liver lysate with ab245247 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab245247 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

        Lane 1: Mouse liver lysate 10 µg (Input). 

        Lane 2: ab245247 IP in mouse liver lysate. 

        Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245247 in mouse liver lysate.

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

        Exposure time: 30 seconds.

      • Anti-Plasminogen antibody [EPR22406-159] (ab245247)
        Anti-Plasminogen antibody [EPR22406-159] (ab245247)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab245247? Please let us know so that we can cite the reference in this datasheet.

      ab245247 has not yet been referenced specifically in any publications.

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