Overview

  • Product name

  • Description

    Rabbit polyclonal to Plastin L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Zebrafish
  • Immunogen

    Recombinant fragment within Zebrafish Plastin L aa 74-404. The exact sequence is proprietary.
    Database link: Q6P698

  • Positive control

    • Zebrafish kidney tissue lysate; zebrafish tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab210099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Suggested antigen retrieval using 1mM EDTA-NaOH (pH8.0) 95°C, 1 Hour

WB 1/500 - 1/3000. Predicted molecular weight: 70 kDa.

Target

  • Function

    Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
  • Tissue specificity

    Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T lymphocytes, neutrophils, monocytes, B lymphocytes, and myeloid cells.
  • Sequence similarities

    Contains 2 actin-binding domains.
    Contains 4 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
  • Post-translational
    modifications

    Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
    The N-terminus is blocked.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell junction. Cell projection. Cell projection > ruffle membrane. Relocalizes to the immunological synapse between peripheral blood T lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at membrane ruffles (By similarity). Relocalizes to actin-rich cell projections upon serine phosphorylation.
  • Information by UniProt
  • Database links

    • Alternative names

      • CP64 antibody
      • L plastin antibody
      • L-plastin antibody
      • Larval cuticle protein 1 antibody
      • LC64P antibody
      • LCP-1 antibody
      • LCP1 antibody
      • LPL antibody
      • Lplastin antibody
      • Lymphocyte cytosolic protein 1 antibody
      • Plastin 2 antibody
      • Plastin-2 antibody
      • PLS2 antibody
      • PLSL_HUMAN antibody
      see all

    Images

    • Anti-Plastin L antibody (ab210099) at 1/1000 dilution + Zebrafish kidney whole cell lysate at 30 µg

      Predicted band size: 70 kDa



      7.5% SDS PAGE

    • Immunohistochemical analysis of paraffin embedded Zebrafish tissue, labeling Plastin L using ab210099 at 1/300 dilution.

    References

    ab210099 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Nothobranchius furzeri Tissue sections (brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization
    Yes - TBS
    Specification
    brain
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 22°C
    Fixative
    Formaldehyde

    Jolien Van houcke

    Verified customer

    Submitted Jul 04 2019

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