Anti-Plastin L antibody (ab210099)
Key features and details
- Rabbit polyclonal to Plastin L
- Suitable for: IHC-P, WB
- Reacts with: Zebrafish
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Plastin L antibody
See all Plastin L primary antibodies -
Description
Rabbit polyclonal to Plastin L -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Zebrafish -
Immunogen
Recombinant fragment within Zebrafish Plastin L aa 74-404. The exact sequence is proprietary.
Database link: Q6P698 -
Positive control
- Zebrafish kidney tissue lysate; zebrafish tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Suggested antigen retrieval using 1mM EDTA-NaOH (pH8.0) 95°C, 1 Hour |
|
WB |
1/500 - 1/3000. Predicted molecular weight: 70 kDa.
|
Notes |
---|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using 1mM EDTA-NaOH (pH8.0) 95°C, 1 Hour |
WB
1/500 - 1/3000. Predicted molecular weight: 70 kDa. |
Target
-
Function
Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69. -
Tissue specificity
Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T lymphocytes, neutrophils, monocytes, B lymphocytes, and myeloid cells. -
Sequence similarities
Contains 2 actin-binding domains.
Contains 4 CH (calponin-homology) domains.
Contains 2 EF-hand domains. -
Post-translational
modificationsPhosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
The N-terminus is blocked. -
Cellular localization
Cytoplasm > cytoskeleton. Cell junction. Cell projection. Cell projection > ruffle membrane. Relocalizes to the immunological synapse between peripheral blood T lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at membrane ruffles (By similarity). Relocalizes to actin-rich cell projections upon serine phosphorylation. - Information by UniProt
-
Database links
- Entrez Gene: 30583 Zebrafish
- SwissProt: Q6P698 Zebrafish
- Unigene: 25823 Zebrafish
-
Alternative names
- CP64 antibody
- L plastin antibody
- L-plastin antibody
see all
Images
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab210099 has been referenced in 1 publication.
- Takaki KK et al. Schistosoma mansoni Eggs Modulate the Timing of Granuloma Formation to Promote Transmission. Cell Host Microbe 29:58-67.e5 (2021). PubMed: 33120115