Product nameAnti-Plastin L antibody
See all Plastin L primary antibodies
DescriptionRabbit polyclonal to Plastin L
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant full length protein corresponding to Human Plastin L aa 2-627.
ARGSVSDEEMMELREAFAKVDTDGNGYISFNELNDLFKAACLPLPGYRVR EITENLMATGDLDQDGRISFDEFIKIFHGLKSTDVAKTFRKAINKKEGIC AIGGTSEQSSVGTQHSYSEEEKYAFVNWINKALENDPDCRHVIPMNPNTN DLFNAVGDGIVLCKMINLSVPDTIDERTINKKKLTPFTIQENLNLALNSA SAIGCHVVNIGAEDLKEGKPYLVLGLLWQVIKIGLFADIELSRNEALIAL LREGESLEDLMKLSPEELLLRWANYHLENAGCNKIGNFSTDIKDSKAYYH LLEQVAPKGDEEGVPAVVIDMSGLREKDDIQRAECMLQQAERLGCRQFVT ATDVVRGNPKLNLAFIANLFNRYPALHKPENQDIDWGALEGETREERTFR NWMNSLGVNPRVNHLYSDLSDALVIFQLYEKIKVPVDWNRVNKPPYPKLG GNMKKLENCNYAVELGKNQAKFSLVGIGGQDLNEGNRTLTLALIWQLMRR YTLNILEEIGGGQKVNDDIIVNWVNETLREAKKSSSISSFKDPKISTSLP VLDLIDAIQPGSINYDLLKTENLNDDEKLNNAKYAISMARKIGARVYALP EDLVEVNPKMVMTVFACLMGKGMKRV
Database link: P13796-1
- WB: HL-60, THP-1, K562, Jurkat, HeLa and NIH/3T3 whole cell lysates. IHC-P: Human spleen tissue. IP: Jurkat whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 70 kDa.|
|IP||1/200 - 1/2000.|
|IHC-P||1/200 - 1/500.|
FunctionActin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
Tissue specificityDetected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T lymphocytes, neutrophils, monocytes, B lymphocytes, and myeloid cells.
Sequence similaritiesContains 2 actin-binding domains.
Contains 4 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
modificationsPhosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
The N-terminus is blocked.
Cellular localizationCytoplasm > cytoskeleton. Cell junction. Cell projection. Cell projection > ruffle membrane. Relocalizes to the immunological synapse between peripheral blood T lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at membrane ruffles (By similarity). Relocalizes to actin-rich cell projections upon serine phosphorylation.
- Information by UniProt
- CP64 antibody
- L plastin antibody
- L-plastin antibody
All lanes : Anti-Plastin L antibody (ab236104) at 1/1000 dilution
Lane 1 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 6 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 70 kDa
Paraffin-embedded human spleen tissue stained for Plastin L using ab236104 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
Plastin L was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate using ab236104 at 1/200 dilution.
Lane 1: Rabbit control IgG in Jurkat whole cell lysate.
Lane 2: ab236104 in Jurkat whole cell lysate.
Lane 3: Jurkat whole cell lysate (20 µg input).
For western blotting an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
ab236104 has not yet been referenced specifically in any publications.