Key features and details
- Rabbit polyclonal to Plastin L
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Plastin L antibody
See all Plastin L primary antibodies
DescriptionRabbit polyclonal to Plastin L
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant full length protein corresponding to Human Plastin L aa 2-627.
ARGSVSDEEMMELREAFAKVDTDGNGYISFNELNDLFKAACLPLPGYRVR EITENLMATGDLDQDGRISFDEFIKIFHGLKSTDVAKTFRKAINKKEGIC AIGGTSEQSSVGTQHSYSEEEKYAFVNWINKALENDPDCRHVIPMNPNTN DLFNAVGDGIVLCKMINLSVPDTIDERTINKKKLTPFTIQENLNLALNSA SAIGCHVVNIGAEDLKEGKPYLVLGLLWQVIKIGLFADIELSRNEALIAL LREGESLEDLMKLSPEELLLRWANYHLENAGCNKIGNFSTDIKDSKAYYH LLEQVAPKGDEEGVPAVVIDMSGLREKDDIQRAECMLQQAERLGCRQFVT ATDVVRGNPKLNLAFIANLFNRYPALHKPENQDIDWGALEGETREERTFR NWMNSLGVNPRVNHLYSDLSDALVIFQLYEKIKVPVDWNRVNKPPYPKLG GNMKKLENCNYAVELGKNQAKFSLVGIGGQDLNEGNRTLTLALIWQLMRR YTLNILEEIGGGQKVNDDIIVNWVNETLREAKKSSSISSFKDPKISTSLP VLDLIDAIQPGSINYDLLKTENLNDDEKLNNAKYAISMARKIGARVYALP EDLVEVNPKMVMTVFACLMGKGMKRV
Database link: P13796-1
- WB: HL-60, THP-1, K562, Jurkat, HeLa and NIH/3T3 whole cell lysates. IHC-P: Human spleen tissue. IP: Jurkat whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 70 kDa.|
|IP||1/200 - 1/2000.|
|IHC-P||1/200 - 1/500.|
FunctionActin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
Tissue specificityDetected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T lymphocytes, neutrophils, monocytes, B lymphocytes, and myeloid cells.
Sequence similaritiesContains 2 actin-binding domains.
Contains 4 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
modificationsPhosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
The N-terminus is blocked.
Cellular localizationCytoplasm > cytoskeleton. Cell junction. Cell projection. Cell projection > ruffle membrane. Relocalizes to the immunological synapse between peripheral blood T lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at membrane ruffles (By similarity). Relocalizes to actin-rich cell projections upon serine phosphorylation.
- Information by UniProt
- CP64 antibody
- L plastin antibody
- L-plastin antibody
All lanes : Anti-Plastin L antibody (ab236104) at 1/1000 dilution
Lane 1 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 6 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 70 kDa
Paraffin-embedded human spleen tissue stained for Plastin L using ab236104 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
Plastin L was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate using ab236104 at 1/200 dilution.
Lane 1: Rabbit control IgG in Jurkat whole cell lysate.
Lane 2: ab236104 in Jurkat whole cell lysate.
Lane 3: Jurkat whole cell lysate (20 µg input).
For western blotting an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236104 has not yet been referenced specifically in any publications.