Overview

  • Product name

    Anti-Plastin L antibody [EPR4277]
    See all Plastin L primary antibodies
  • Description

    Rabbit monoclonal [EPR4277] to Plastin L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Plastin L aa 150-250. The exact sequence is proprietary.

  • Positive control

    • THP1, K562 and Jurkat whole cell lysate (ab7899); Human tonsil tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 70 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C allow to cool for 10-20 min).
ICC 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
    • Tissue specificity

      Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T lymphocytes, neutrophils, monocytes, B lymphocytes, and myeloid cells.
    • Sequence similarities

      Contains 2 actin-binding domains.
      Contains 4 CH (calponin-homology) domains.
      Contains 2 EF-hand domains.
    • Post-translational
      modifications

      Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
      The N-terminus is blocked.
    • Cellular localization

      Cytoplasm > cytoskeleton. Cell junction. Cell projection. Cell projection > ruffle membrane. Relocalizes to the immunological synapse between peripheral blood T lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at membrane ruffles (By similarity). Relocalizes to actin-rich cell projections upon serine phosphorylation.
    • Information by UniProt
    • Database links

    • Alternative names

      • CP64 antibody
      • L plastin antibody
      • L-plastin antibody
      • Larval cuticle protein 1 antibody
      • LC64P antibody
      • LCP-1 antibody
      • LCP1 antibody
      • LPL antibody
      • Lplastin antibody
      • Lymphocyte cytosolic protein 1 antibody
      • Plastin 2 antibody
      • Plastin-2 antibody
      • PLS2 antibody
      • PLSL_HUMAN antibody
      see all

    Images

    • All lanes : Anti-Plastin L antibody [EPR4277] (ab109124) at 1/1000 dilution

      Lane 1 : THP1 cell lysates
      Lane 2 : K562 cell lysates
      Lane 3 : Jurkat cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 70 kDa

    • ab109124, at 1/100 dilution staining Plastin L in paraffin-embedded Human tonsil tissue, by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab109124 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab109124.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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