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    platelet-antibody-ab53304.pdf

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Cardiovascular Blood Platelets
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Anti-Platelet antibody (ab53304)

  • Datasheet
  • SDS
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Abpromise

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Find out more.

Key features and details

  • Sheep polyclonal to Platelet
  • Suitable for: ELISA
  • Reacts with: Rabbit
  • Isotype: IgG

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Secondary
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Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)

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Overview

  • Product name

    Anti-Platelet antibody
    See all Platelet primary antibodies
  • Description

    Sheep polyclonal to Platelet
  • Host species

    Sheep
  • Specificity

    ab53304 reacts with lapine platelets. Binding to platelets is strongly positive with thymocyte-absorbed anti platelet serum and negative with thymocyte-absorbed normal sheep serum.
  • Tested applications

    Suitable for: ELISAmore details
  • Species reactivity

    Reacts with: Rabbit
  • Immunogen

    Rabbit platelets

  • General notes

    This product should be stored undiluted. Should this product contain a precipitate microcentrifugation before use is recommended.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: Whole serum, EDTA

    Defibrinated plasma containing EDTA used to chelate excess calcium used to reverse citrate anticoagulation, pH neutralized with sodium hydroxide.
  • Concentration information loading...
  • Purification notes

    Defibrinated plasma containing EDTA used to chelate excess calcium used to reverse citrate anticoagulation, pH neutralized with sodium hydroxide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets

Associated products

  • Isotype control

    • Sheep IgG - Isotype Control (ab37385)

Applications

Our Abpromise guarantee covers the use of ab53304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Target

  • Relevance

    Like red blood cells, platelets are anuclear (no cell nucleus) and discoid (disc shaped); they measure 1.5 to 3.0 µm in diameter. The body has a very limited reserve of platelets, so they can be rapidly depleted. They contain RNA, a canalicular system, and several different types of granules; lysosomes (containing acid hydrolases), dense bodies (containing ADP, ATP serotonin and calcium) and alpha granules (containing fibrinogen, factor V, vitronectin, thrombospondin and von Willebrand factor), the contents of which are released upon activation of the platelet. These granule contents play an important role in both hemostasis and in the inflammatory response. Platelets or thrombocytes are the cell fragments circulating in the blood that are involved in the cellular mechanisms of primary hemostasis leading to the formation of blood clots. Dysfunction or low levels of platelets predisposes to bleeding, while high levels, although usually asymptomatic, may increase the risk of thrombosis. Platelets are produced in the bone marrow; the progenitor cell for platelets is the megakaryocyte. It is about twelve times larger than an erythrocyte, possesses a lobed nucleus and sheds platelets into the circulation.
  • Cellular localization

    Cell Membrane

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab53304? Please let us know so that we can cite the reference in this datasheet.

    ab53304 has been referenced in 1 publication.

    • Lan Y  et al. Engulfment of platelets delays endothelial cell aging via girdin and its phosphorylation. Int J Mol Med 42:988-997 (2018). PubMed: 29786109

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