Overview

  • Product name

    Anti-PLB1 antibody
  • Description

    Mouse polyclonal to PLB1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full-length Human PLB1 protein (AAH65041)

  • Positive control

    • Human colon lysate and PLB1 transfected 293T lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab92915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 500 - 1000 µg/ml. Predicted molecular weight: 163 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      PLB1 is a membrane-associated phospholipase which exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. It shows preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. It also exhibits esterase activity toward p-nitrophenyl. It may act on the brush border membrane to facilitate the absorption of digested lipids.
    • Cellular localization

      Apical cell membrane; Single-pass type I membrane protein. Note: Present in the intestinal brush border membranes.
    • Database links

    • Alternative names

      • hPLB antibody
      • Lysophospholipase antibody
      • Phospholipase A2 antibody
      • Phospholipase B antibody
      • Phospholipase B/lipase antibody
      • Phospholipase B1 antibody
      • Phospholipase B1 membrane associated antibody
      • PLB 1 antibody
      • PLB antibody
      • PLB/LIP antibody
      see all

    Images

    • Anti-PLB1 antibody (ab92915) at 1/500 dilution + Human colon lysate at 50 µg

      Predicted band size: 163 kDa

    References

    This product has been referenced in:

    • Campos-Garcia L  et al. Candida albicans and non-albicans Isolates from Bloodstream Have Different Capacities to Induce Neutrophil Extracellular Traps. J Fungi (Basel) 5:N/A (2019). Read more (PubMed: 30939757) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Answer

    Thank you for your enquiry. Please rest assured that these 2 products were made from the same immunogen just that ab90553 is the unpurified version, and ab92915 has been purified. 1) ab90553 is a polyclonal antibody. We injected the immunogen (PLB1 full-length human protein) into mouse and obtained its sera. 2) ab92915 is produced by the same method, but after getting the sera, we further purify it by protein A-Sepharose method and quantify its concentration. Since ab92915 is a better version of PLB1 mouse polyclonal antibody, we will not be stocking any more of the unpurified version. I hope this information will be helpful. If there is anything else that I can help you with, please do not hesitate to contact me.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up