Overview

  • Product name

  • Description

    Rabbit polyclonal to PLBD2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Rabbit, Cow, Dog, Human, Chimpanzee, Zebrafish, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Mouse PLBD2 aa 500 to the C-terminus conjugated to keyhole limpet haemocyanin.
    Database link: Q3TCN2

  • Positive control

    • This antibody gave a positive signal in both Rat Spleen and Lung tissue lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab138334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 65 kDa).

Target

  • Function

    Putative phospholipase.
  • Tissue specificity

    Ubiquitously expressed, with highest levels in heart, brain and liver.
  • Sequence similarities

    Belongs to the phospholipase B-like family.
  • Post-translational
    modifications

    The p76 protein is synthesized as a 80 kDa precursor which is then processed into a N-terminal 32 kDa form and a C-terminal 45 kDa form.
    Glycosylated; contains mannose 6-phosphate sugars.
  • Cellular localization

    Lysosome lumen.
  • Information by UniProt
  • Database links

  • Alternative names

    • 76 kDa protein antibody
    • LAMA-like protein 2 antibody
    • Lamina ancestor homolog 2 antibody
    • p76 antibody
    • Phospholipase B domain-containing protein 2 antibody
    • Plbd2 antibody
    • PLBL2_HUMAN antibody
    • Putative phospholipase B-like 2 45 kDa form antibody
    see all

Images

  • All lanes : Anti-PLBD2 antibody (ab138334) at 1 µg/ml

    Lane 1 : Spleen (Rat) Tissue Lysate
    Lane 2 : Lung (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 65 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 15 kDa (possible cleavage fragment), 40 kDa (possible cleavage fragment)


    Exposure time: 6 minutes


    The band observed at 40 kDa could potentially be a cleaved form of PLBD2, representing the 346 amino acid chain called "Putative phospholipase B-like 2 40 kDa form".

    The band observed at 15 kDa could potentially be a cleaved form of PLBD2, representing the 81 amino acid chain called "Putative phospholipase B-like 2 15 kDa form".

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab138334 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab138334 has not yet been referenced specifically in any publications.

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