Product nameAnti-PLBD2 antibody
See all PLBD2 primary antibodies
DescriptionRabbit polyclonal to PLBD2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Rabbit, Cow, Dog, Human, Chimpanzee, Zebrafish, Macaque monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide corresponding to Mouse PLBD2 aa 500 to the C-terminus conjugated to keyhole limpet haemocyanin.
Database link: Q3TCN2
- This antibody gave a positive signal in both Rat Spleen and Lung tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab138334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 65 kDa).|
Tissue specificityUbiquitously expressed, with highest levels in heart, brain and liver.
Sequence similaritiesBelongs to the phospholipase B-like family.
modificationsThe p76 protein is synthesized as a 80 kDa precursor which is then processed into a N-terminal 32 kDa form and a C-terminal 45 kDa form.
Glycosylated; contains mannose 6-phosphate sugars.
Cellular localizationLysosome lumen.
- Information by UniProt
- 76 kDa protein antibody
- LAMA-like protein 2 antibody
- Lamina ancestor homolog 2 antibody
All lanes : Anti-PLBD2 antibody (ab138334) at 1 µg/ml
Lane 1 : Spleen (Rat) Tissue Lysate
Lane 2 : Lung (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa (possible cleavage fragment), 40 kDa (possible cleavage fragment)
Exposure time: 6 minutes
The band observed at 40 kDa could potentially be a cleaved form of PLBD2, representing the 346 amino acid chain called "Putative phospholipase B-like 2 40 kDa form".
The band observed at 15 kDa could potentially be a cleaved form of PLBD2, representing the 81 amino acid chain called "Putative phospholipase B-like 2 15 kDa form".
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab138334 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab138334 has not yet been referenced specifically in any publications.