Overview

  • Product name

    Anti-PLBD2 antibody [EPR21204]
    See all PLBD2 primary antibodies
  • Description

    Rabbit monoclonal [EPR21204] to PLBD2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human PLBD2 aa 400-550.
    Database link: Q8NHP8

  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

Properties

Applications

Our Abpromise guarantee covers the use of ab225580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB 1/50000. Predicted molecular weight: 65 kDa.

Target

  • Function

    Putative phospholipase.
  • Tissue specificity

    Ubiquitously expressed, with highest levels in heart, brain and liver.
  • Sequence similarities

    Belongs to the phospholipase B-like family.
  • Post-translational
    modifications

    The p76 protein is synthesized as a 80 kDa precursor which is then processed into a N-terminal 32 kDa form and a C-terminal 45 kDa form.
    Glycosylated; contains mannose 6-phosphate sugars.
  • Cellular localization

    Lysosome lumen.
  • Information by UniProt
  • Database links

  • Alternative names

    • 76 kDa protein antibody
    • LAMA-like protein 2 antibody
    • Lamina ancestor homolog 2 antibody
    • p76 antibody
    • Phospholipase B domain-containing protein 2 antibody
    • Plbd2 antibody
    • PLBL2_HUMAN antibody
    • Putative phospholipase B-like 2 45 kDa form antibody
    see all

Images

  • All lanes : Anti-PLBD2 antibody [EPR21204] (ab225580) at 1 µg/ml

    Lane 1 : Human heart tissue lysate
    Lane 2 : Human cerebellum tissue lysate
    Lane 3 : Mouse cerebellum tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 65 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% BSA solution before being incubated with ab225580 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • Different concentrations of immobilised PLBD2 protein was recognised in an indirect ELISA by ab225580. Bound ab225580 (1 µg x mL-1) was detected by peroxidase conjugated goat anti-rabbit IgG (0.16 µg x mL-1). OD (background signal subtracted) shown on the y-axis.

References

ab225580 has not yet been referenced specifically in any publications.

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