Product nameAnti-PLBD2 antibody [EPR21204]
See all PLBD2 primary antibodies
DescriptionRabbit monoclonal [EPR21204] to PLBD2
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human PLBD2 aa 400-550.
Database link: Q8NHP8
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
- Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
Our Abpromise guarantee covers the use of ab225580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||1/50000. Predicted molecular weight: 65 kDa.|
Tissue specificityUbiquitously expressed, with highest levels in heart, brain and liver.
Sequence similaritiesBelongs to the phospholipase B-like family.
modificationsThe p76 protein is synthesized as a 80 kDa precursor which is then processed into a N-terminal 32 kDa form and a C-terminal 45 kDa form.
Glycosylated; contains mannose 6-phosphate sugars.
Cellular localizationLysosome lumen.
- Information by UniProt
- 76 kDa protein antibody
- LAMA-like protein 2 antibody
- Lamina ancestor homolog 2 antibody
All lanes : Anti-PLBD2 antibody [EPR21204] (ab225580) at 1 µg/ml
Lane 1 : Human heart tissue lysate
Lane 2 : Human cerebellum tissue lysate
Lane 3 : Mouse cerebellum tissue lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% BSA solution before being incubated with ab225580 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Different concentrations of immobilised PLBD2 protein was recognised in an indirect ELISA by ab225580. Bound ab225580 (1 µg x mL-1) was detected by peroxidase conjugated goat anti-rabbit IgG (0.16 µg x mL-1). OD (background signal subtracted) shown on the y-axis.
ab225580 has not yet been referenced specifically in any publications.