Overview

  • Product name

    Anti-PLBD2 antibody [EPR21742]
    See all PLBD2 primary antibodies
  • Description

    Rabbit monoclonal [EPR21742] to PLBD2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PLBD2 aa 150-250. The exact sequence is proprietary.
    Database link: Q8NHP8

  • Positive control

    • WB: U937, MCF7, MEF and NIH/3T3 whole cell lysates; human fetal spleen, fetal kidney and fetal brain tissue lysates; Mouse brain, kidney and liver tissue lysates; Rat brain, liver and spleen tissue lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab216866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 65 kDa.

Target

  • Function

    Putative phospholipase.
  • Tissue specificity

    Ubiquitously expressed, with highest levels in heart, brain and liver.
  • Sequence similarities

    Belongs to the phospholipase B-like family.
  • Post-translational
    modifications

    The p76 protein is synthesized as a 80 kDa precursor which is then processed into a N-terminal 32 kDa form and a C-terminal 45 kDa form.
    Glycosylated; contains mannose 6-phosphate sugars.
  • Cellular localization

    Lysosome lumen.
  • Information by UniProt
  • Database links

  • Alternative names

    • 76 kDa protein antibody
    • LAMA-like protein 2 antibody
    • Lamina ancestor homolog 2 antibody
    • p76 antibody
    • Phospholipase B domain-containing protein 2 antibody
    • Plbd2 antibody
    • PLBL2_HUMAN antibody
    • Putative phospholipase B-like 2 45 kDa form antibody
    see all

Images

  • All lanes : Anti-PLBD2 antibody [EPR21742] (ab216866) at 1/1000 dilution

    Lane 1 : U937 (human histiocytic lymphoma cell line) whole cell lysate at 20 µg
    Lane 2 : Human fetal spleen tissue lysate at 20 µg
    Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 4 : MEF (mouse embryonic fibroblast cell line) whole cell lysate at 20 µg
    Lane 5 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate
    Lane 6 : Human fetal kidney tissue lysate at 20 µg
    Lane 7 : Human fetal brain tissue lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 65 kDa
    Observed band size: 76 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 32/28 kDa (possible cleavage fragment)



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1 & 2: 59 seconds

    Lane 3: 15 seconds.

    Lanes 4 & 5: 3 seconds.

    Lanes 6 & 7: 3 minutes.

    Full-length PLBD2 (76 kDa) can be cleaved into N-terminal and C-terminal fragments according to the literature (PMID:17007843, PMID: 17105447).

    This antibody recognizes both full-length and the N-terminal fragment of PLBD2.

  • All lanes : Anti-PLBD2 antibody [EPR21742] (ab216866) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Mouse liver tissue lysate
    Lane 4 : Rat brain tissue lysate
    Lane 5 : Rat liver tissue lysate
    Lane 6 : Rat spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 65 kDa
    Observed band size: 76 kDa why is the actual band size different from the predicted?
    Additional bands at: 32/28 kDa (possible cleavage fragment)



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1-3: 6 seconds.

    Lanes 4-6: 26 seconds.

    Full-length PLBD2 (76 kDa) can be cleaved into N-terminal and C-terminal fragments according to the literature (PMID:17007843, PMID: 17105447).

    This antibody recognizes both full-length and the N-terminal fragment of PLBD2.

References

ab216866 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab216866.
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