Product nameAnti-PLBD2 antibody [EPR21742] - BSA and Azide free
See all PLBD2 primary antibodies
DescriptionRabbit monoclonal [EPR21742] to PLBD2 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PLBD2 aa 150-250. The exact sequence is proprietary.
Database link: Q8NHP8
- WB: U937, MCF7, MEF and NIH/3T3 whole cell lysates; human fetal spleen, fetal kidney and fetal brain tissue lysates.
Ab231825 is the carrier-free version of ab216866. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab231825 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab231825 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 65 kDa.|
Tissue specificityUbiquitously expressed, with highest levels in heart, brain and liver.
Sequence similaritiesBelongs to the phospholipase B-like family.
modificationsThe p76 protein is synthesized as a 80 kDa precursor which is then processed into a N-terminal 32 kDa form and a C-terminal 45 kDa form.
Glycosylated; contains mannose 6-phosphate sugars.
Cellular localizationLysosome lumen.
- Information by UniProt
- 76 kDa protein antibody
- LAMA-like protein 2 antibody
- Lamina ancestor homolog 2 antibody
All lanes : Anti-PLBD2 antibody [EPR21742] (ab216866) at 1/1000 dilution
Lane 1 : U937 (human histiocytic lymphoma cell line) whole cell lysate at 20 µg
Lane 2 : Human fetal spleen tissue lysate at 20 µg
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 4 : MEF (mouse embryonic fibroblast cell line) whole cell lysate at 20 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg
Lane 6 : Human fetalkidney tissue lysate at 20 µg
Lane 7 : Human fetal brain tissue lysate at 10 µg
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 65 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted?
Additional bands at: 32/28 kDa (possible cleavage fragment)
Blocking and dilution buffer: 5% NFDM/TBST.
Lanes 1 & 2: 59 seconds
Lane 3: 15 seconds.
Lanes 4 & 5: 3 seconds.
Lanes 6 & 7: 3 minutes.
Full-length PLBD2 (76 kDa) can be cleaved into N-terminal and C-terminal fragments according to the literature (PMID:17007843, PMID: 17105447).
This antibody recognizes both full-length and the N-terminal fragment of PLBD2.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216866).
ab231825 has not yet been referenced specifically in any publications.