Recombinant Anti-PLBD2 antibody [IGX4128H] (ab213295)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [IGX4128H] to PLBD2
- Suitable for: WB, ELISA
- Reacts with: Mouse, Human
Overview
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Product name
Anti-PLBD2 antibody [IGX4128H]
See all PLBD2 primary antibodies -
Description
Human monoclonal [IGX4128H] to PLBD2 -
Host species
Human -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is considered to be commercially sensitive.
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Positive control
- WB: Mouse Cerebellum tissue lysate
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General notes
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant antibody.
Human monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
IGX4128H -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab213295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 65 kDa.
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ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 65 kDa. |
ELISA
Use at an assay dependent concentration. |
Target
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Function
Putative phospholipase. -
Tissue specificity
Ubiquitously expressed, with highest levels in heart, brain and liver. -
Sequence similarities
Belongs to the phospholipase B-like family. -
Post-translational
modificationsThe p76 protein is synthesized as a 80 kDa precursor which is then processed into a N-terminal 32 kDa form and a C-terminal 45 kDa form.
Glycosylated; contains mannose 6-phosphate sugars. -
Cellular localization
Lysosome lumen. - Information by UniProt
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Database links
- Entrez Gene: 196463 Human
- Entrez Gene: 71772 Mouse
- SwissProt: Q8NHP8 Human
- SwissProt: Q3TCN2 Mouse
- Unigene: 115896 Human
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Alternative names
- 76 kDa protein antibody
- LAMA-like protein 2 antibody
- Lamina ancestor homolog 2 antibody
see all
Images
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Different concentrations of immobilised recombinant fragment of human PLBD2 was recognised in an indirect ELISA by ab213295 (1 µg x mL-1). The antibody was bound by peroxidase conjugated recombinant protein A at 0.2 µg x mL-1. OD (background signal subtracted) shown on the y-axis.
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Anti-PLBD2 antibody [IGX4128H] (ab213295) at 1 µg/ml + Mouse Cerebellum Tissue Lysate at 10 µg
Secondary
HRP conjugated Goat Anti-Human IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Additional bands at: 42 kDa (possible cleavage fragment)
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab213295 overnight at 4°C. Antibody binding was detected using an anti-human antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab213295 has not yet been referenced specifically in any publications.