Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [IGX4128H] to PLBD2
- Suitable for: WB, ELISA
- Reacts with: Mouse, Human
Product nameAnti-PLBD2 antibody [IGX4128H]
See all PLBD2 primary antibodies
DescriptionHuman monoclonal [IGX4128H] to PLBD2
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment. This information is considered to be commercially sensitive.
- WB: Mouse Cerebellum tissue lysate
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant antibody.
Human monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituents: 0.484% Tris buffer, 0.45% Glycine, 40% Glycerol, 1.044% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab213295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 65 kDa.|
|ELISA||Use at an assay dependent concentration.|
Tissue specificityUbiquitously expressed, with highest levels in heart, brain and liver.
Sequence similaritiesBelongs to the phospholipase B-like family.
modificationsThe p76 protein is synthesized as a 80 kDa precursor which is then processed into a N-terminal 32 kDa form and a C-terminal 45 kDa form.
Glycosylated; contains mannose 6-phosphate sugars.
Cellular localizationLysosome lumen.
- Information by UniProt
- 76 kDa protein antibody
- LAMA-like protein 2 antibody
- Lamina ancestor homolog 2 antibody
Different concentrations of immobilised recombinant fragment of human PLBD2 was recognised in an indirect ELISA by ab213295 (1 µg x mL-1). The antibody was bound by peroxidase conjugated recombinant protein A at 0.2 µg x mL-1. OD (background signal subtracted) shown on the y-axis.
Anti-PLBD2 antibody [IGX4128H] (ab213295) at 1 µg/ml + Mouse Cerebellum Tissue Lysate at 10 µg
HRP conjugated Goat Anti-Human IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Additional bands at: 42 kDa (possible cleavage fragment)
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab213295 overnight at 4°C. Antibody binding was detected using an anti-human antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab213295 has not yet been referenced specifically in any publications.