Overview

  • Product name

    Anti-PLBD2 antibody [IGX4128H]
    See all PLBD2 primary antibodies
  • Description

    Human monoclonal [IGX4128H] to PLBD2
  • Host species

    Human
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Mouse Cerebellum tissue lysate
  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant antibody. 
    Human monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: 0.484% Tris buffer, 0.45% Glycine, 40% Glycerol, 1.044% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    IGX4128H
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab213295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 65 kDa.
ELISA Use at an assay dependent concentration.

Target

  • Function

    Putative phospholipase.
  • Tissue specificity

    Ubiquitously expressed, with highest levels in heart, brain and liver.
  • Sequence similarities

    Belongs to the phospholipase B-like family.
  • Post-translational
    modifications

    The p76 protein is synthesized as a 80 kDa precursor which is then processed into a N-terminal 32 kDa form and a C-terminal 45 kDa form.
    Glycosylated; contains mannose 6-phosphate sugars.
  • Cellular localization

    Lysosome lumen.
  • Information by UniProt
  • Database links

  • Alternative names

    • 76 kDa protein antibody
    • LAMA-like protein 2 antibody
    • Lamina ancestor homolog 2 antibody
    • p76 antibody
    • Phospholipase B domain-containing protein 2 antibody
    • Plbd2 antibody
    • PLBL2_HUMAN antibody
    • Putative phospholipase B-like 2 45 kDa form antibody
    see all

Images

  •  Different concentrations of immobilised recombinant fragment of human PLBD2 was recognised in an indirect ELISA by ab213295 (1 µg x mL-1). The antibody was bound by peroxidase conjugated recombinant protein A at 0.2 µg x mL-1. OD (background signal subtracted) shown on the y-axis.
  • Anti-PLBD2 antibody [IGX4128H] (ab213295) at 1 µg/ml + Mouse Cerebellum Tissue Lysate at 10 µg

    Secondary
    HRP conjugated Goat Anti-Human IgG (H+L) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 65 kDa
    Additional bands at: 42 kDa (possible cleavage fragment)


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab213295 overnight at 4°C. Antibody binding was detected using an anti-human antibody conjugated to HRP, and visualised using ECL development solution ab133406.

References

ab213295 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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