Overview

  • Product name

  • Description

    Rabbit polyclonal to PLC-delta-3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 349-589 of Human PLC-delta-3 (UniProt: Q8N3E9).

  • Positive control

    • HCT116 whole cell lysate; Human Hepatoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab151594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 89 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Essential for trophoblast and placental development. May participate in cytokinesis by hydrolyzing PIP2 at the cleavage furrow.
  • Tissue specificity

    Present in corneal epithelial cells (at protein level).
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 3 EF-hand domains.
    Contains 1 PH domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
  • Domain

    The C2 domain is a Ca(2+)-dependent membrane-targeting module.
    The PH domain mediates interaction with the surface membrane by binding to PIP2.
  • Cellular localization

    Membrane. Cytoplasm. Cleavage furrow. Localizes at the cleavage furrow during cytokinesis.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1-phosphatidylinositol-4 antibody
    • 5-bisphosphate phosphodiesterase delta-3 antibody
    • Phosphoinositide phospholipase C-delta-3 antibody
    • Phospholipase C-delta-3 antibody
    • PLC-delta-3 antibody
    • PLCD3 antibody
    • PLCD3_HUMAN antibody
    see all

Images

  • Anti-PLC-delta-3 antibody (ab151594) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size: 89 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human Hepatoma tissue, labeling PLC-delta-3 with ab151594 at 1/500 dilution.

References

ab151594 has not yet been referenced specifically in any publications.

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