Key features and details
- Rabbit polyclonal to PLCD1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PLCD1 antibody
See all PLCD1 primary antibodies
DescriptionRabbit polyclonal to PLCD1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
Recombinant fragment corresponding to a region within amino acids 503-750 of Human PLCD1 (UniProt P51178).
- A549, HeLa, HepG2 and HCT116 cell lysates; HeLa cells; Human breast carcinoma tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 86 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).|
|ICC/IF||1/100 - 1/1000.|
RelevancePLCD1 acts as a signal transducer that generates 2 second messengers, diacylglycerol and inositol 1,4,5-trisphosphate, by hydrolyzing inositol phospholipids. The PLC family of enzymes are diverse and vary in structure and tissue distribution.
- 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta 1 antibody
- NDNC3 antibody
- Phosphoinositide phospholipase C antibody
Anti-PLCD1 antibody (ab154610) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 86 kDa
7.5% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PLCD1 with ab154610 at 1/250 dilution.
Immunofluorescent analysis of methanol-fixed HeLa cells labeling PLCD1 with ab154610 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
ab154610 has been referenced in 2 publications.
- Hörer S et al. A Monoallelic Two-Hit Mechanism in PLCD1 Explains the Genetic Pathogenesis of Hereditary Trichilemmal Cyst Formation. J Invest Dermatol 139:2154-2163.e5 (2019). PubMed: 31082376
- Béziau DM et al. Expression of phosphoinositide-specific phospholipase C isoforms in native endothelial cells. PLoS One 10:e0123769 (2015). WB, IHC-P . PubMed: 25875657