Anti-PLCD1 antibody (ab154610)
Key features and details
- Rabbit polyclonal to PLCD1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PLCD1 antibody
See all PLCD1 primary antibodies -
Description
Rabbit polyclonal to PLCD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
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Immunogen
Recombinant fragment corresponding to a region within amino acids 503-750 of Human PLCD1 (UniProt P51178).
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Positive control
- A549, HeLa, HepG2 and HCT116 cell lysates; HeLa cells; Human breast carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 86 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Relevance
PLCD1 acts as a signal transducer that generates 2 second messengers, diacylglycerol and inositol 1,4,5-trisphosphate, by hydrolyzing inositol phospholipids. The PLC family of enzymes are diverse and vary in structure and tissue distribution. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 281986 Cow
- Entrez Gene: 5333 Human
- Entrez Gene: 18799 Mouse
- Entrez Gene: 100627099 Pig
- Entrez Gene: 24655 Rat
- Omim: 602142 Human
- SwissProt: P51178 Human
- SwissProt: Q8R3B1 Mouse
see all -
Alternative names
- 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta 1 antibody
- NDNC3 antibody
- Phosphoinositide phospholipase C antibody
see all
Images
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Western blot - Anti-PLCD1 antibody (ab154610)Anti-PLCD1 antibody (ab154610) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 86 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCD1 antibody (ab154610)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PLCD1 with ab154610 at 1/250 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-PLCD1 antibody (ab154610)Immunofluorescent analysis of methanol-fixed HeLa cells labeling PLCD1 with ab154610 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
Protocols
References (2)
ab154610 has been referenced in 2 publications.
- Hörer S et al. A Monoallelic Two-Hit Mechanism in PLCD1 Explains the Genetic Pathogenesis of Hereditary Trichilemmal Cyst Formation. J Invest Dermatol 139:2154-2163.e5 (2019). PubMed: 31082376
- Béziau DM et al. Expression of phosphoinositide-specific phospholipase C isoforms in native endothelial cells. PLoS One 10:e0123769 (2015). WB, IHC-P . PubMed: 25875657
