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Rabbit polyclonal to PLCD1
Predicted to work with:
Mouse, Rat, Cow, Pig
Recombinant fragment corresponding to a region within amino acids 503-750 of Human PLCD1 (UniProt P51178).
A549, HeLa, HepG2 and HCT116 cell lysates; HeLa cells; Human breast carcinoma tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 86 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
1/100 - 1/1000.
PLCD1 acts as a signal transducer that generates 2 second messengers, diacylglycerol and inositol 1,4,5-trisphosphate, by hydrolyzing inositol phospholipids. The PLC family of enzymes are diverse and vary in structure and tissue distribution.
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta 1 antibody
Phosphoinositide phospholipase C antibody
Western blot - Anti-PLCD1 antibody (ab154610)
Anti-PLCD1 antibody (ab154610) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 86 kDa 7.5% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCD1 antibody (ab154610)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PLCD1 with ab154610 at 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-PLCD1 antibody (ab154610)
Immunofluorescent analysis of methanol-fixed HeLa cells labeling PLCD1 with ab154610 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"