Anti-PLCD1 antibody (ab154610)

Product name

Anti-PLCD1 antibody
See all PLCD1 primary antibodies
Description

Rabbit polyclonal to PLCD1
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IFmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
Immunogen

Recombinant fragment corresponding to a region within amino acids 503-750 of Human PLCD1 (UniProt P51178).
Positive control
- A549, HeLa, HepG2 and HCT116 cell lysates; HeLa cells; Human breast carcinoma tissue.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab154610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/500 - 1/3000. Predicted molecular weight: 86 kDa.
IHC-P		1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF		1/100 - 1/1000.

Relevance

PLCD1 acts as a signal transducer that generates 2 second messengers, diacylglycerol and inositol 1,4,5-trisphosphate, by hydrolyzing inositol phospholipids. The PLC family of enzymes are diverse and vary in structure and tissue distribution.
Cellular localization

Cytoplasmic
Database links
- Entrez Gene: 281986 Cow
- Entrez Gene: 5333 Human
- Entrez Gene: 18799 Mouse
- Entrez Gene: 100627099 Pig
- Entrez Gene: 24655 Rat
- Omim: 602142 Human
- SwissProt: P51178 Human
- SwissProt: Q8R3B1 Mouse
- SwissProt: P10688 Rat
- Unigene: 80776 Human
- Unigene: 23963 Mouse
- Unigene: 12324 Rat
see all
Alternative names
- 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta 1 antibody
- NDNC3 antibody
- Phosphoinositide phospholipase C antibody
- Phosphoinositide phospholipase C delta 1 antibody
- Phospholipase C delta 1 antibody
- Phospholipase C III antibody
- PLC delta 1 antibody
- PLC III antibody
see all

Western blot - Anti-PLCD1 antibody (ab154610)

Anti-PLCD1 antibody (ab154610) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

Predicted band size: 86 kDa

7.5% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCD1 antibody (ab154610)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PLCD1 with ab154610 at 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-PLCD1 antibody (ab154610)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling PLCD1 with ab154610 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

Béziau DM et al. Expression of phosphoinositide-specific phospholipase C isoforms in native endothelial cells. PLoS One 10:e0123769 (2015). WB, IHC-P . Read more (PubMed: 25875657) »

Publishing research using ab154610? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As