Anti-PLCD1 antibody (ab154610)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-PLCD1 antibody
See all PLCD1 primary antibodies -
DescriptionRabbit polyclonal to PLCD1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
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Immunogen
Recombinant fragment corresponding to a region within amino acids 503-750 of Human PLCD1 (UniProt P51178).
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Positive control
- A549, HeLa, HepG2 and HCT116 cell lysates; HeLa cells; Human breast carcinoma tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 86 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). | |
| ICC/IF | 1/100 - 1/1000. |
Target
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RelevancePLCD1 acts as a signal transducer that generates 2 second messengers, diacylglycerol and inositol 1,4,5-trisphosphate, by hydrolyzing inositol phospholipids. The PLC family of enzymes are diverse and vary in structure and tissue distribution.
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Cellular localizationCytoplasmic
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Database links
- Entrez Gene: 281986 Cow
- Entrez Gene: 5333 Human
- Entrez Gene: 18799 Mouse
- Entrez Gene: 100627099 Pig
- Entrez Gene: 24655 Rat
- Omim: 602142 Human
- SwissProt: P51178 Human
- SwissProt: Q8R3B1 Mouse
see all -
Alternative names
- 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta 1 antibody
- NDNC3 antibody
- Phosphoinositide phospholipase C antibody
see all
Images
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Western blot - Anti-PLCD1 antibody (ab154610)Anti-PLCD1 antibody (ab154610) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 86 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCD1 antibody (ab154610)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PLCD1 with ab154610 at 1/250 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-PLCD1 antibody (ab154610)Immunofluorescent analysis of methanol-fixed HeLa cells labeling PLCD1 with ab154610 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
Protocols
References
This product has been referenced in:
- Béziau DM et al. Expression of phosphoinositide-specific phospholipase C isoforms in native endothelial cells. PLoS One 10:e0123769 (2015). WB, IHC-P . Read more (PubMed: 25875657) »
