Recombinant Anti-PLCD1 antibody [EPR8430] (ab134936)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8430] to PLCD1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PLCD1 antibody [EPR8430]
See all PLCD1 primary antibodies -
Description
Rabbit monoclonal [EPR8430] to PLCD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PLCD1 aa 200-300. The exact sequence is proprietary.
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Positive control
- Human brain and testis tissues; HeLa and Human fetal lung lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8430 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 86 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For antigen retrieval: heat up to 98°C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended. |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 86 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For antigen retrieval: heat up to 98°C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended. |
Target
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Relevance
PLCD1 acts as a signal transducer that generates 2 second messengers, diacylglycerol and inositol 1,4,5-trisphosphate, by hydrolyzing inositol phospholipids. The PLC family of enzymes are diverse and vary in structure and tissue distribution. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 5333 Human
- Omim: 602142 Human
- SwissProt: P51178 Human
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Alternative names
- 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta 1 antibody
- NDNC3 antibody
- Phosphoinositide phospholipase C antibody
see all
Images
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All lanes : Anti-PLCD1 antibody [EPR8430] (ab134936) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human fetal lung tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 86 kDa -
Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human testis tissue, labelling PLCD1 using ab134936 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human brain tissue, labelling PLCD1 using ab134931 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab134936 has been referenced in 3 publications.
- Swarup V et al. Identification of Conserved Proteomic Networks in Neurodegenerative Dementia. Cell Rep 31:107807 (2020). PubMed: 32579933
- Tanaka M et al. IQGAP1 activates PLC-d1 by direct binding and moving along microtubule with DLC-1 to cell surface. FASEB Bioadv 1:465-480 (2019). PubMed: 32123844
- Shao Q et al. Phospholipase Cd1 suppresses cell migration and invasion of breast cancer cells by modulating KIF3A-mediated ERK1/2/ß- catenin/MMP7 signalling. Oncotarget 8:29056-29066 (2017). WB, IHC . PubMed: 28423710