Overview

  • Product name

    Anti-PLCD1 antibody [EPR8430]
    See all PLCD1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8430] to PLCD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IP, WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PLCD1 aa 200-300. The exact sequence is proprietary.

  • Positive control

    • Human brain and testis tissues; HeLa and Human fetal lung lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8430
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/10 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 86 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For antigen retrieval: heat up to 98°C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Relevance

      PLCD1 acts as a signal transducer that generates 2 second messengers, diacylglycerol and inositol 1,4,5-trisphosphate, by hydrolyzing inositol phospholipids. The PLC family of enzymes are diverse and vary in structure and tissue distribution.
    • Cellular localization

      Cytoplasmic
    • Database links

    • Alternative names

      • 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta 1 antibody
      • NDNC3 antibody
      • Phosphoinositide phospholipase C antibody
      • Phosphoinositide phospholipase C delta 1 antibody
      • Phospholipase C delta 1 antibody
      • Phospholipase C III antibody
      • PLC delta 1 antibody
      • PLC III antibody
      see all

    Images

    • All lanes : Anti-PLCD1 antibody [EPR8430] (ab134936) at 1/1000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : Human fetal lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 86 kDa

    • Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human testis tissue, labelling PLCD1 using ab134936 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human brain tissue, labelling PLCD1 using ab134931 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Shao Q  et al. Phospholipase Cd1 suppresses cell migration and invasion of breast cancer cells by modulating KIF3A-mediated ERK1/2/ß- catenin/MMP7 signalling. Oncotarget 8:29056-29066 (2017). WB, IHC . Read more (PubMed: 28423710) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134936.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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