Product nameAnti-PLCD4 antibody
See all PLCD4 primary antibodies
DescriptionMouse polyclonal to PLCD4
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Pig, Macaque monkey, Orangutan
- PLCD4 transfected 293T cell lysate. HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Our Abpromise guarantee covers the use of ab169169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 88 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionHydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca(2+) mobilization in the zona pellucida-induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation.
Tissue specificityHighly expressed in skeletal muscle and kidney tissues, and at moderate level in intestinal tissue. Expressed in corneal epithelial cells.
Sequence similaritiesContains 1 C2 domain.
Contains 3 EF-hand domains.
Contains 1 PH domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
DomainThe PDZ-binding motif mediates the interaction with GRIP1.
The C2 domain mediates pre-localization to the membrane prior to Ca(2+) import and non-selective Ca(2+)-mediated targeting to various cellular membranes.
The PH domain is not a critical determinant of the membrane localization.
Cellular localizationMembrane. Nucleus. Cytoplasm. Endoplasmic reticulum. Localizes primarily to intracellular membranes mostly to the endoplasmic reticulum.
- Information by UniProt
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase delta 4 antibody
- 1-phosphatidylinositol 4 antibody
- 5-bisphosphate phosphodiesterase delta-4 antibody
All lanes : Anti-PLCD4 antibody (ab169169) at 1/500 dilution
Lane 1 : PLCD4 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
All lanes : Goat anti-mouse HRP at 1/2500 dilution
Predicted band size: 88 kDa
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling PLCD4 with ab169169 at 10μg/ml.
ab169169 has not yet been referenced specifically in any publications.